Detailed information of TA system TA043465

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4724214..4724816
• Replicon: chromosome
• Accession: NZ_AP027197
• Organism: Escherichia coli strain 02E060

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: QMG97_RS24435
• Protein ID: WP_000897305.1
• Coordinates: 4724505..4724816 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: QMG97_RS24430
• Protein ID: WP_000356397.1
• Coordinates: 4724214..4724504 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QMG97_RS24405 (4719760)	4719760..4720395	+	636	WP_000829008.1	formate dehydrogenase cytochrome b556 subunit	-
QMG97_RS24410 (4720392)	4720392..4721321	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
QMG97_RS24415 (4721721)	4721721..4722701	+	981	WP_000399685.1	IS110-like element IS621 family transposase	-
QMG97_RS24420 (4722930)	4722930..4723172	-	243	WP_001086390.1	protein YiiF	-
QMG97_RS24425 (4723391)	4723391..4723609	-	219	WP_001295676.1	CopG family transcriptional regulator	-
QMG97_RS24430 (4724214)	4724214..4724504	-	291	WP_000356397.1	NadS family protein	Antitoxin
QMG97_RS24435 (4724505)	4724505..4724816	-	312	WP_000897305.1	hypothetical protein	Toxin
QMG97_RS24440 (4725045)	4725045..4725953	+	909	WP_001162704.1	alpha/beta hydrolase	-
QMG97_RS24445 (4726017)	4726017..4726958	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
QMG97_RS24450 (4727003)	4727003..4727440	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
QMG97_RS24455 (4727437)	4727437..4728309	-	873	WP_000920762.1	virulence factor BrkB family protein	-
QMG97_RS24460 (4728303)	4728303..4728902	-	600	WP_001315111.1	glucose-1-phosphatase	-
QMG97_RS24465 (4729001)	4729001..4729786	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	4721721..4722701	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T43465 WP_000897305.1 NZ_AP027197:c4724816-4724505 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T43465 NZ_AP027197:c4724816-4724505 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT43465 WP_000356397.1 NZ_AP027197:c4724504-4724214 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT43465 NZ_AP027197:c4724504-4724214 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7