Detailed information of TA system TA043461

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3745630..3746357
• Replicon: chromosome
• Accession: NZ_AP027197
• Organism: Escherichia coli strain 02E060

Toxin (Protein)

• Gene name: higB
• Uniprot ID: I2UEW4
• Locus tag: QMG97_RS19685
• Protein ID: WP_000547559.1
• Coordinates: 3746046..3746357 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: QMG97_RS19680
• Protein ID: WP_000126294.1
• Coordinates: 3745630..3746049 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QMG97_RS19660 (3740680)	3740680..3741207	-	528	WP_001078781.1	electron transport protein HydN	-
QMG97_RS19665 (3741356)	3741356..3742366	-	1011	WP_001377591.1	DNA-binding transcriptional regulator AscG	-
QMG97_RS19670 (3742626)	3742626..3744083	+	1458	WP_001107856.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
QMG97_RS19675 (3744092)	3744092..3745516	+	1425	WP_000110313.1	6-phospho-beta-glucosidase AscB	-
QMG97_RS19680 (3745630)	3745630..3746049	-	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
QMG97_RS19685 (3746046)	3746046..3746357	-	312	WP_000547559.1	type II toxin-antitoxin system HigB family toxin	Toxin
QMG97_RS19690 (3746531)	3746531..3747274	-	744	WP_001341829.1	hypothetical protein	-
QMG97_RS19695 (3747317)	3747317..3747787	-	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
QMG97_RS19700 (3747780)	3747780..3748190	-	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
QMG97_RS19705 (3748187)	3748187..3748954	-	768	WP_000067401.1	formate hydrogenlyase subunit HycG	-
QMG97_RS19710 (3748954)	3748954..3749496	-	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
QMG97_RS19715 (3749506)	3749506..3751215	-	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (24-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12450.11 Da
• Isoelectric Point: 9.7249

>T43461 WP_000547559.1 NZ_AP027197:c3746357-3746046 [Escherichia coli]
MHIISKAPFEESARKYPNDALAHQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T43461 NZ_AP027197:c3746357-3746046 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCATCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT43461 WP_000126294.1 NZ_AP027197:c3746049-3745630 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT43461 NZ_AP027197:c3746049-3745630 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCTGTT
GGCGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A0F3V163