Detailed information of TA system TA043424

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4834888..4835490
• Replicon: chromosome
• Accession: NZ_AP027191
• Organism: Escherichia coli strain 10153

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: QMG89_RS25240
• Protein ID: WP_000897305.1
• Coordinates: 4835179..4835490 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: QMG89_RS25235
• Protein ID: WP_000356397.1
• Coordinates: 4834888..4835178 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QMG89_RS25210 (4830813)	4830813..4831715	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
QMG89_RS25215 (4831712)	4831712..4832347	+	636	WP_000829008.1	formate dehydrogenase cytochrome b556 subunit	-
QMG89_RS25220 (4832344)	4832344..4833273	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
QMG89_RS25225 (4833604)	4833604..4833846	-	243	WP_001086390.1	protein YiiF	-
QMG89_RS25230 (4834065)	4834065..4834283	-	219	WP_001295676.1	CopG family transcriptional regulator	-
QMG89_RS25235 (4834888)	4834888..4835178	-	291	WP_000356397.1	NadS family protein	Antitoxin
QMG89_RS25240 (4835179)	4835179..4835490	-	312	WP_000897305.1	hypothetical protein	Toxin
QMG89_RS25245 (4835719)	4835719..4836627	+	909	WP_001162704.1	alpha/beta hydrolase	-
QMG89_RS25250 (4836691)	4836691..4837632	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
QMG89_RS25255 (4837677)	4837677..4838114	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
QMG89_RS25260 (4838111)	4838111..4838983	-	873	WP_000920762.1	virulence factor BrkB family protein	-
QMG89_RS25265 (4838977)	4838977..4839576	-	600	WP_001315111.1	glucose-1-phosphatase	-
QMG89_RS25270 (4839675)	4839675..4840460	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T43424 WP_000897305.1 NZ_AP027191:c4835490-4835179 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T43424 NZ_AP027191:c4835490-4835179 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT43424 WP_000356397.1 NZ_AP027191:c4835178-4834888 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT43424 NZ_AP027191:c4835178-4834888 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7