Detailed information of TA system TA043420

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3857440..3858167
• Replicon: chromosome
• Accession: NZ_AP027191
• Organism: Escherichia coli strain 10153

Toxin (Protein)

• Gene name: higB
• Uniprot ID: I2UEW4
• Locus tag: QMG89_RS20480
• Protein ID: WP_000547559.1
• Coordinates: 3857856..3858167 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: QMG89_RS20475
• Protein ID: WP_000126294.1
• Coordinates: 3857440..3857859 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QMG89_RS20455 (3852490)	3852490..3853017	-	528	WP_001078781.1	electron transport protein HydN	-
QMG89_RS20460 (3853166)	3853166..3854176	-	1011	WP_001377591.1	DNA-binding transcriptional regulator AscG	-
QMG89_RS20465 (3854436)	3854436..3855893	+	1458	WP_001107856.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
QMG89_RS20470 (3855902)	3855902..3857326	+	1425	WP_000110313.1	6-phospho-beta-glucosidase AscB	-
QMG89_RS20475 (3857440)	3857440..3857859	-	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
QMG89_RS20480 (3857856)	3857856..3858167	-	312	WP_000547559.1	type II toxin-antitoxin system HigB family toxin	Toxin
QMG89_RS20485 (3858341)	3858341..3859084	-	744	WP_001341829.1	hypothetical protein	-
QMG89_RS20490 (3859127)	3859127..3859597	-	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
QMG89_RS20495 (3859590)	3859590..3860000	-	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
QMG89_RS20500 (3859997)	3859997..3860764	-	768	WP_000067401.1	formate hydrogenlyase subunit HycG	-
QMG89_RS20505 (3860764)	3860764..3861306	-	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
QMG89_RS20510 (3861316)	3861316..3863025	-	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (24-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12450.11 Da
• Isoelectric Point: 9.7249

>T43420 WP_000547559.1 NZ_AP027191:c3858167-3857856 [Escherichia coli]
MHIISKAPFEESARKYPNDALAHQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T43420 NZ_AP027191:c3858167-3857856 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCATCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT43420 WP_000126294.1 NZ_AP027191:c3857859-3857440 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT43420 NZ_AP027191:c3857859-3857440 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCTGTT
GGCGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A0F3V163