Detailed information of TA system TA043383

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3794047..3794774
• Replicon: chromosome
• Accession: NZ_AP027188
• Organism: Escherichia coli strain PV0838

Toxin (Protein)

• Gene name: higB
• Uniprot ID: I2UEW4
• Locus tag: QMG68_RS20050
• Protein ID: WP_000547559.1
• Coordinates: 3794463..3794774 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: QMG68_RS20045
• Protein ID: WP_000126294.1
• Coordinates: 3794047..3794466 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QMG68_RS20025 (3789097)	3789097..3789624	-	528	WP_001078781.1	electron transport protein HydN	-
QMG68_RS20030 (3789773)	3789773..3790783	-	1011	WP_001377591.1	DNA-binding transcriptional regulator AscG	-
QMG68_RS20035 (3791043)	3791043..3792500	+	1458	WP_001107856.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
QMG68_RS20040 (3792509)	3792509..3793933	+	1425	WP_000110313.1	6-phospho-beta-glucosidase AscB	-
QMG68_RS20045 (3794047)	3794047..3794466	-	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
QMG68_RS20050 (3794463)	3794463..3794774	-	312	WP_000547559.1	type II toxin-antitoxin system HigB family toxin	Toxin
QMG68_RS20055 (3794948)	3794948..3795691	-	744	WP_001341829.1	hypothetical protein	-
QMG68_RS20060 (3795734)	3795734..3796204	-	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
QMG68_RS20065 (3796197)	3796197..3796607	-	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
QMG68_RS20070 (3796604)	3796604..3797371	-	768	WP_000067401.1	formate hydrogenlyase subunit HycG	-
QMG68_RS20075 (3797371)	3797371..3797913	-	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
QMG68_RS20080 (3797923)	3797923..3799632	-	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (24-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12450.11 Da
• Isoelectric Point: 9.7249

>T43383 WP_000547559.1 NZ_AP027188:c3794774-3794463 [Escherichia coli]
MHIISKAPFEESARKYPNDALAHQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T43383 NZ_AP027188:c3794774-3794463 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCATCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT43383 WP_000126294.1 NZ_AP027188:c3794466-3794047 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT43383 NZ_AP027188:c3794466-3794047 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCTGTT
GGCGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A0F3V163