Detailed information of TA system TA043352

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4773328..4773930
• Replicon: chromosome
• Accession: NZ_AP027185
• Organism: Escherichia coli strain NIID080884

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: QMG74_RS24570
• Protein ID: WP_000897305.1
• Coordinates: 4773619..4773930 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: QMG74_RS24565
• Protein ID: WP_000356397.1
• Coordinates: 4773328..4773618 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QMG74_RS24540 (4768874)	4768874..4769509	+	636	WP_000829008.1	formate dehydrogenase cytochrome b556 subunit	-
QMG74_RS24545 (4769506)	4769506..4770435	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
QMG74_RS24550 (4770835)	4770835..4771815	+	981	WP_000399685.1	IS110-like element IS621 family transposase	-
QMG74_RS24555 (4772044)	4772044..4772286	-	243	WP_001086390.1	protein YiiF	-
QMG74_RS24560 (4772505)	4772505..4772723	-	219	WP_001295676.1	CopG family transcriptional regulator	-
QMG74_RS24565 (4773328)	4773328..4773618	-	291	WP_000356397.1	NadS family protein	Antitoxin
QMG74_RS24570 (4773619)	4773619..4773930	-	312	WP_000897305.1	hypothetical protein	Toxin
QMG74_RS24575 (4774159)	4774159..4775067	+	909	WP_001162704.1	alpha/beta hydrolase	-
QMG74_RS24580 (4775131)	4775131..4776072	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
QMG74_RS24585 (4776117)	4776117..4776554	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
QMG74_RS24590 (4776551)	4776551..4777423	-	873	WP_000920762.1	virulence factor BrkB family protein	-
QMG74_RS24595 (4777417)	4777417..4778016	-	600	WP_001315111.1	glucose-1-phosphatase	-
QMG74_RS24600 (4778115)	4778115..4778900	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	4770835..4771815	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T43352 WP_000897305.1 NZ_AP027185:c4773930-4773619 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T43352 NZ_AP027185:c4773930-4773619 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT43352 WP_000356397.1 NZ_AP027185:c4773618-4773328 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT43352 NZ_AP027185:c4773618-4773328 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7