Detailed information of TA system TA043318

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4769603..4770205
• Replicon: chromosome
• Accession: NZ_AP027181
• Organism: Escherichia coli strain 98E11

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: QMG82_RS24810
• Protein ID: WP_000897305.1
• Coordinates: 4769894..4770205 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: QMG82_RS24805
• Protein ID: WP_000356397.1
• Coordinates: 4769603..4769893 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QMG82_RS24780 (4765149)	4765149..4765784	+	636	WP_000829008.1	formate dehydrogenase cytochrome b556 subunit	-
QMG82_RS24785 (4765781)	4765781..4766710	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
QMG82_RS24790 (4767110)	4767110..4768090	+	981	WP_000399648.1	IS110-like element IS621 family transposase	-
QMG82_RS24795 (4768319)	4768319..4768561	-	243	WP_001086390.1	protein YiiF	-
QMG82_RS24800 (4768780)	4768780..4768998	-	219	WP_001295676.1	CopG family transcriptional regulator	-
QMG82_RS24805 (4769603)	4769603..4769893	-	291	WP_000356397.1	NadS family protein	Antitoxin
QMG82_RS24810 (4769894)	4769894..4770205	-	312	WP_000897305.1	hypothetical protein	Toxin
QMG82_RS24815 (4770434)	4770434..4771342	+	909	WP_001162704.1	alpha/beta hydrolase	-
QMG82_RS24820 (4771406)	4771406..4772347	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
QMG82_RS24825 (4772392)	4772392..4772829	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
QMG82_RS24830 (4772826)	4772826..4773698	-	873	WP_000920762.1	virulence factor BrkB family protein	-
QMG82_RS24835 (4773692)	4773692..4774291	-	600	WP_001315111.1	glucose-1-phosphatase	-
QMG82_RS24840 (4774390)	4774390..4775175	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	4767110..4768090	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T43318 WP_000897305.1 NZ_AP027181:c4770205-4769894 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T43318 NZ_AP027181:c4770205-4769894 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT43318 WP_000356397.1 NZ_AP027181:c4769893-4769603 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT43318 NZ_AP027181:c4769893-4769603 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7