Detailed information of TA system TA043314

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3713203..3713930
• Replicon: chromosome
• Accession: NZ_AP027181
• Organism: Escherichia coli strain 98E11

Toxin (Protein)

• Gene name: higB
• Uniprot ID: I2UEW4
• Locus tag: QMG82_RS19460
• Protein ID: WP_000547559.1
• Coordinates: 3713619..3713930 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: QMG82_RS19455
• Protein ID: WP_000126294.1
• Coordinates: 3713203..3713622 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QMG82_RS19435 (3708253)	3708253..3708780	-	528	WP_001078781.1	electron transport protein HydN	-
QMG82_RS19440 (3708929)	3708929..3709939	-	1011	WP_001377591.1	DNA-binding transcriptional regulator AscG	-
QMG82_RS19445 (3710199)	3710199..3711656	+	1458	WP_001107856.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
QMG82_RS19450 (3711665)	3711665..3713089	+	1425	WP_000110313.1	6-phospho-beta-glucosidase AscB	-
QMG82_RS19455 (3713203)	3713203..3713622	-	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
QMG82_RS19460 (3713619)	3713619..3713930	-	312	WP_000547559.1	type II toxin-antitoxin system HigB family toxin	Toxin
QMG82_RS19465 (3714104)	3714104..3714847	-	744	WP_001341829.1	hypothetical protein	-
QMG82_RS19470 (3714890)	3714890..3715360	-	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
QMG82_RS19475 (3715353)	3715353..3715763	-	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
QMG82_RS19480 (3715760)	3715760..3716527	-	768	WP_000067401.1	formate hydrogenlyase subunit HycG	-
QMG82_RS19485 (3716527)	3716527..3717069	-	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
QMG82_RS19490 (3717079)	3717079..3718788	-	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (24-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12450.11 Da
• Isoelectric Point: 9.7249

>T43314 WP_000547559.1 NZ_AP027181:c3713930-3713619 [Escherichia coli]
MHIISKAPFEESARKYPNDALAHQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T43314 NZ_AP027181:c3713930-3713619 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCATCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT43314 WP_000126294.1 NZ_AP027181:c3713622-3713203 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT43314 NZ_AP027181:c3713622-3713203 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCTGTT
GGCGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A0F3V163