Detailed information of TA system TA043274

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 5111353..5111955
• Replicon: chromosome
• Accession: NZ_AP027176
• Organism: Escherichia coli strain EH2252

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: QMG71_RS26305
• Protein ID: WP_000897305.1
• Coordinates: 5111353..5111664 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: QMG71_RS26310
• Protein ID: WP_000356397.1
• Coordinates: 5111665..5111955 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QMG71_RS26275 (5106383)	5106383..5107168	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
QMG71_RS26280 (5107267)	5107267..5107866	+	600	WP_001315111.1	glucose-1-phosphatase	-
QMG71_RS26285 (5107860)	5107860..5108732	+	873	WP_000920762.1	virulence factor BrkB family protein	-
QMG71_RS26290 (5108729)	5108729..5109166	+	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
QMG71_RS26295 (5109211)	5109211..5110152	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
QMG71_RS26300 (5110216)	5110216..5111124	-	909	WP_001162704.1	alpha/beta hydrolase	-
QMG71_RS26305 (5111353)	5111353..5111664	+	312	WP_000897305.1	hypothetical protein	Toxin
QMG71_RS26310 (5111665)	5111665..5111955	+	291	WP_000356397.1	NadS family protein	Antitoxin
QMG71_RS26315 (5112560)	5112560..5112778	+	219	WP_001295676.1	CopG family transcriptional regulator	-
QMG71_RS26320 (5112997)	5112997..5113239	+	243	WP_001086390.1	protein YiiF	-
QMG71_RS26325 (5113570)	5113570..5114499	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
QMG71_RS26330 (5114496)	5114496..5115131	-	636	WP_000829008.1	formate dehydrogenase cytochrome b556 subunit	-
QMG71_RS26335 (5115128)	5115128..5116030	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T43274 WP_000897305.1 NZ_AP027176:5111353-5111664 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T43274 NZ_AP027176:5111353-5111664 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT43274 WP_000356397.1 NZ_AP027176:5111665-5111955 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT43274 NZ_AP027176:5111665-5111955 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7