Detailed information of TA system TA043270

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3782142..3782869
• Replicon: chromosome
• Accession: NZ_AP027176
• Organism: Escherichia coli strain EH2252

Toxin (Protein)

• Gene name: higB
• Uniprot ID: I2UEW4
• Locus tag: QMG71_RS19845
• Protein ID: WP_000547559.1
• Coordinates: 3782558..3782869 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: QMG71_RS19840
• Protein ID: WP_000126294.1
• Coordinates: 3782142..3782561 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QMG71_RS19820 (3777192)	3777192..3777719	-	528	WP_001078781.1	electron transport protein HydN	-
QMG71_RS19825 (3777868)	3777868..3778878	-	1011	WP_001377591.1	DNA-binding transcriptional regulator AscG	-
QMG71_RS19830 (3779138)	3779138..3780595	+	1458	WP_024017722.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
QMG71_RS19835 (3780604)	3780604..3782028	+	1425	WP_000110313.1	6-phospho-beta-glucosidase AscB	-
QMG71_RS19840 (3782142)	3782142..3782561	-	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
QMG71_RS19845 (3782558)	3782558..3782869	-	312	WP_000547559.1	type II toxin-antitoxin system HigB family toxin	Toxin
QMG71_RS19850 (3783043)	3783043..3783786	-	744	WP_001341829.1	hypothetical protein	-
QMG71_RS19855 (3783829)	3783829..3784299	-	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
QMG71_RS19860 (3784292)	3784292..3784702	-	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
QMG71_RS19865 (3784699)	3784699..3785466	-	768	WP_000067401.1	formate hydrogenlyase subunit HycG	-
QMG71_RS19870 (3785466)	3785466..3786008	-	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
QMG71_RS19875 (3786018)	3786018..3787727	-	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (24-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12450.11 Da
• Isoelectric Point: 9.7249

>T43270 WP_000547559.1 NZ_AP027176:c3782869-3782558 [Escherichia coli]
MHIISKAPFEESARKYPNDALAHQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T43270 NZ_AP027176:c3782869-3782558 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCATCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT43270 WP_000126294.1 NZ_AP027176:c3782561-3782142 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT43270 NZ_AP027176:c3782561-3782142 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCTGTT
GGCGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A0F3V163