Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 268774..269309 Replicon chromosome
Accession NZ_AP027176
Organism Escherichia coli strain EH2252

Toxin (Protein)


Gene name yafQ Uniprot ID A0A0F3UZU8
Locus tag QMG71_RS01220 Protein ID WP_032183258.1
Coordinates 268774..269061 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag QMG71_RS01225 Protein ID WP_000729704.1
Coordinates 269049..269309 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QMG71_RS01205 (266356) 266356..266934 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
QMG71_RS01210 (267140) 267140..267907 + 768 WP_000333380.1 class II glutamine amidotransferase -
QMG71_RS01215 (267878) 267878..268618 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
QMG71_RS01220 (268774) 268774..269061 - 288 WP_032183258.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
QMG71_RS01225 (269049) 269049..269309 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
QMG71_RS01230 (269495) 269495..270268 + 774 WP_000543897.1 C40 family peptidase -
QMG71_RS01235 (270325) 270325..270681 - 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
QMG71_RS01240 (270674) 270674..270952 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
QMG71_RS01245 (271057) 271057..273150 - 2094 WP_001140806.1 flagellar biosynthesis protein FlhA -
QMG71_RS01250 (273134) 273134..274273 - 1140 WP_000785656.1 flagellar type III secretion system protein FlhB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(7-94)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11166.96 Da        Isoelectric Point: 10.2267

>T43243 WP_032183258.1 NZ_AP027176:c269061-268774 [Escherichia coli]
IRNLNQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDK
LLRFERTGTHAALFG

Download         Length: 288 bp

>T43243 NZ_AP027176:c269061-268774 [Escherichia coli]
ATTAGGAATTTAAATCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAA
GGATATGAATAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACC
CGCTGCAAGGTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAA
CTTTTACGATTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT43243 WP_000729704.1 NZ_AP027176:c269309-269049 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT43243 NZ_AP027176:c269309-269049 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0F3UZU8

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References