Detailed information of TA system TA043230

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 5010432..5011034
• Replicon: chromosome
• Accession: NZ_AP027170
• Organism: Escherichia coli strain 20.1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: QMG87_RS25680
• Protein ID: WP_000897305.1
• Coordinates: 5010432..5010743 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: QMG87_RS25685
• Protein ID: WP_000356397.1
• Coordinates: 5010744..5011034 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QMG87_RS25650 (5005462)	5005462..5006247	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
QMG87_RS25655 (5006346)	5006346..5006945	+	600	WP_001315111.1	glucose-1-phosphatase	-
QMG87_RS25660 (5006939)	5006939..5007811	+	873	WP_000920762.1	virulence factor BrkB family protein	-
QMG87_RS25665 (5007808)	5007808..5008245	+	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
QMG87_RS25670 (5008290)	5008290..5009231	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
QMG87_RS25675 (5009295)	5009295..5010203	-	909	WP_001162704.1	alpha/beta hydrolase	-
QMG87_RS25680 (5010432)	5010432..5010743	+	312	WP_000897305.1	hypothetical protein	Toxin
QMG87_RS25685 (5010744)	5010744..5011034	+	291	WP_000356397.1	NadS family protein	Antitoxin
QMG87_RS25690 (5011639)	5011639..5011857	+	219	WP_001295676.1	CopG family transcriptional regulator	-
QMG87_RS25695 (5012076)	5012076..5012318	+	243	WP_001086390.1	protein YiiF	-
QMG87_RS25700 (5012649)	5012649..5013578	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
QMG87_RS25705 (5013575)	5013575..5014210	-	636	WP_000829008.1	formate dehydrogenase cytochrome b556 subunit	-
QMG87_RS25710 (5014207)	5014207..5015109	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T43230 WP_000897305.1 NZ_AP027170:5010432-5010743 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T43230 NZ_AP027170:5010432-5010743 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT43230 WP_000356397.1 NZ_AP027170:5010744-5011034 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT43230 NZ_AP027170:5010744-5011034 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7