Detailed information of TA system TA043226

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3695491..3696218
• Replicon: chromosome
• Accession: NZ_AP027170
• Organism: Escherichia coli strain 20.1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: I2UEW4
• Locus tag: QMG87_RS19295
• Protein ID: WP_000547559.1
• Coordinates: 3695907..3696218 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: QMG87_RS19290
• Protein ID: WP_000126294.1
• Coordinates: 3695491..3695910 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QMG87_RS19270 (3690541)	3690541..3691068	-	528	WP_001078781.1	electron transport protein HydN	-
QMG87_RS19275 (3691217)	3691217..3692227	-	1011	WP_001377591.1	DNA-binding transcriptional regulator AscG	-
QMG87_RS19280 (3692487)	3692487..3693944	+	1458	WP_024017722.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
QMG87_RS19285 (3693953)	3693953..3695377	+	1425	WP_000110313.1	6-phospho-beta-glucosidase AscB	-
QMG87_RS19290 (3695491)	3695491..3695910	-	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
QMG87_RS19295 (3695907)	3695907..3696218	-	312	WP_000547559.1	type II toxin-antitoxin system HigB family toxin	Toxin
QMG87_RS19300 (3696392)	3696392..3697135	-	744	WP_001341829.1	hypothetical protein	-
QMG87_RS19305 (3697178)	3697178..3697648	-	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
QMG87_RS19310 (3697641)	3697641..3698051	-	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
QMG87_RS19315 (3698048)	3698048..3698815	-	768	WP_000067401.1	formate hydrogenlyase subunit HycG	-
QMG87_RS19320 (3698815)	3698815..3699357	-	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
QMG87_RS19325 (3699367)	3699367..3701076	-	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (24-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12450.11 Da
• Isoelectric Point: 9.7249

>T43226 WP_000547559.1 NZ_AP027170:c3696218-3695907 [Escherichia coli]
MHIISKAPFEESARKYPNDALAHQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T43226 NZ_AP027170:c3696218-3695907 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCATCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT43226 WP_000126294.1 NZ_AP027170:c3695910-3695491 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT43226 NZ_AP027170:c3695910-3695491 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCTGTT
GGCGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A0F3V163