Detailed information of TA system TA043189

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4878418..4879020
• Replicon: chromosome
• Accession: NZ_AP027165
• Organism: Escherichia coli strain H19

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: QMG77_RS24790
• Protein ID: WP_000897305.1
• Coordinates: 4878418..4878729 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: QMG77_RS24795
• Protein ID: WP_000356397.1
• Coordinates: 4878730..4879020 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QMG77_RS24760 (4873448)	4873448..4874233	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
QMG77_RS24765 (4874332)	4874332..4874931	+	600	WP_001315111.1	glucose-1-phosphatase	-
QMG77_RS24770 (4874925)	4874925..4875797	+	873	WP_000920762.1	virulence factor BrkB family protein	-
QMG77_RS24775 (4875794)	4875794..4876231	+	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
QMG77_RS24780 (4876276)	4876276..4877217	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
QMG77_RS24785 (4877281)	4877281..4878189	-	909	WP_001162704.1	alpha/beta hydrolase	-
QMG77_RS24790 (4878418)	4878418..4878729	+	312	WP_000897305.1	hypothetical protein	Toxin
QMG77_RS24795 (4878730)	4878730..4879020	+	291	WP_000356397.1	NadS family protein	Antitoxin
QMG77_RS24800 (4879625)	4879625..4879843	+	219	WP_001295676.1	CopG family transcriptional regulator	-
QMG77_RS24805 (4880062)	4880062..4880304	+	243	WP_001086390.1	protein YiiF	-
QMG77_RS24810 (4880533)	4880533..4881513	-	981	WP_000399648.1	IS110-like element IS621 family transposase	-
QMG77_RS24815 (4881913)	4881913..4882842	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
QMG77_RS24820 (4882839)	4882839..4883474	-	636	WP_000829008.1	formate dehydrogenase cytochrome b556 subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	4880533..4881513	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T43189 WP_000897305.1 NZ_AP027165:4878418-4878729 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T43189 NZ_AP027165:4878418-4878729 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT43189 WP_000356397.1 NZ_AP027165:4878730-4879020 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT43189 NZ_AP027165:4878730-4879020 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7