Detailed information of TA system TA043185

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3551079..3551806
• Replicon: chromosome
• Accession: NZ_AP027165
• Organism: Escherichia coli strain H19

Toxin (Protein)

• Gene name: higB
• Uniprot ID: I2UEW4
• Locus tag: QMG77_RS18340
• Protein ID: WP_000547559.1
• Coordinates: 3551495..3551806 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: QMG77_RS18335
• Protein ID: WP_000126294.1
• Coordinates: 3551079..3551498 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QMG77_RS18315 (3546129)	3546129..3546656	-	528	WP_001078781.1	electron transport protein HydN	-
QMG77_RS18320 (3546805)	3546805..3547815	-	1011	WP_001377591.1	DNA-binding transcriptional regulator AscG	-
QMG77_RS18325 (3548075)	3548075..3549532	+	1458	WP_024017722.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
QMG77_RS18330 (3549541)	3549541..3550965	+	1425	WP_000110313.1	6-phospho-beta-glucosidase AscB	-
QMG77_RS18335 (3551079)	3551079..3551498	-	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
QMG77_RS18340 (3551495)	3551495..3551806	-	312	WP_000547559.1	type II toxin-antitoxin system HigB family toxin	Toxin
QMG77_RS18345 (3551980)	3551980..3552723	-	744	WP_001341829.1	hypothetical protein	-
QMG77_RS18350 (3552766)	3552766..3553236	-	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
QMG77_RS18355 (3553229)	3553229..3553639	-	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
QMG77_RS18360 (3553636)	3553636..3554403	-	768	WP_000067401.1	formate hydrogenlyase subunit HycG	-
QMG77_RS18365 (3554403)	3554403..3554945	-	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
QMG77_RS18370 (3554955)	3554955..3556664	-	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (24-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12450.11 Da
• Isoelectric Point: 9.7249

>T43185 WP_000547559.1 NZ_AP027165:c3551806-3551495 [Escherichia coli]
MHIISKAPFEESARKYPNDALAHQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T43185 NZ_AP027165:c3551806-3551495 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCATCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT43185 WP_000126294.1 NZ_AP027165:c3551498-3551079 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT43185 NZ_AP027165:c3551498-3551079 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCTGTT
GGCGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A0F3V163