Detailed information of TA system TA043183

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 2678451..2678953
• Replicon: chromosome
• Accession: NZ_AP027165
• Organism: Escherichia coli strain H19

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: U9YDH0
• Locus tag: QMG77_RS13935
• Protein ID: WP_000767829.1
• Coordinates: 2678451..2678705 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: S1Q063
• Locus tag: QMG77_RS13940
• Protein ID: WP_001259255.1
• Coordinates: 2678702..2678953 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QMG77_RS13905 (2673466)	2673466..2673693	-	228	WP_000985273.1	thiosulfate utilization sulfurtransferase TsuB/YeeD	-
QMG77_RS13910 (2673707)	2673707..2674765	-	1059	WP_000492324.1	thiosulfate utilization transporter TsuA/YeeE	-
QMG77_RS13915 (2674944)	2674944..2676302	-	1359	WP_000019197.1	putrescine/proton symporter PlaP	-
QMG77_RS13920 (2676292)	2676292..2676354	-	63	WP_010723108.1	membrane protein YoeI	-
QMG77_RS13925 (2676569)	2676569..2677498	-	930	WP_000803351.1	LysR substrate-binding domain-containing protein	-
QMG77_RS13930 (2677544)	2677544..2678368	-	825	WP_000754737.1	SDR family oxidoreductase	-
QMG77_RS13935 (2678451)	2678451..2678705	-	255	WP_000767829.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
QMG77_RS13940 (2678702)	2678702..2678953	-	252	WP_001259255.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
QMG77_RS13945 (2679236)	2679236..2679283	+	48	WP_001375657.1	his operon leader peptide	-
QMG77_RS13950 (2679429)	2679429..2680328	+	900	WP_000131782.1	ATP phosphoribosyltransferase	-
QMG77_RS13955 (2680334)	2680334..2681638	+	1305	WP_000009589.1	histidinol dehydrogenase	-
QMG77_RS13960 (2681635)	2681635..2682705	+	1071	WP_000108914.1	histidinol-phosphate transaminase	-
QMG77_RS13965 (2682705)	2682705..2683772	+	1068	WP_000080089.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10183.58 Da
• Isoelectric Point: 8.0353

>T43183 WP_000767829.1 NZ_AP027165:c2678705-2678451 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T43183 NZ_AP027165:c2678705-2678451 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9307.58 Da
• Isoelectric Point: 4.7954

>AT43183 WP_001259255.1 NZ_AP027165:c2678953-2678702 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT43183 NZ_AP027165:c2678953-2678702 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• PDB: 6NY6
• PDB: 2A6S
• PDB: 6OXA
• PDB: 2A6R
• PDB: 4V8X
• PDB: 6OTR
• PDB: 6OXI
• PDB: 2A6Q

Antitoxin

• AlphaFold DB: A0A7U9LNR0