Detailed information of TA system TA043166

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 1785616..1785987
• Replicon: chromosome
• Accession: NZ_AP027165
• Organism: Escherichia coli strain H19

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A0D6ZRD3
• Locus tag: QMG77_RS09260
• Protein ID: WP_001443846.1
• Coordinates: 1785838..1785987 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	1785616..1785794 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QMG77_RS09230 (1781368)	1781368..1781541	+	174	WP_001443847.1	protein YnaL	-
QMG77_RS09235 (1781571)	1781571..1782944	+	1374	WP_000123745.1	ATP-dependent RNA helicase DbpA	-
QMG77_RS09240 (1783073)	1783073..1784008	-	936	WP_001157407.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
QMG77_RS09245 (1784060)	1784060..1785295	-	1236	WP_000040851.1	site-specific integrase	-
QMG77_RS09250 (1785297)	1785297..1785512	-	216	WP_000079604.1	excisionase XisR	-
- (1785616)	1785616..1785794	+	179	NuclAT_0	-	Antitoxin
- (1785616)	1785616..1785794	+	179	NuclAT_0	-	Antitoxin
- (1785616)	1785616..1785794	+	179	NuclAT_0	-	Antitoxin
- (1785616)	1785616..1785794	+	179	NuclAT_0	-	Antitoxin
QMG77_RS09255 (1785612)	1785612..1785800	-	189	WP_001302840.1	DUF1187 family protein	-
QMG77_RS09260 (1785838)	1785838..1785987	-	150	WP_001443846.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
QMG77_RS09265 (1786043)	1786043..1786852	-	810	WP_000166313.1	recombination protein RecT	-
QMG77_RS09270 (1786845)	1786845..1789445	-	2601	WP_000105150.1	exodeoxyribonuclease VIII	-
QMG77_RS09275 (1789547)	1789547..1789822	-	276	WP_000632297.1	protein RacC	-
QMG77_RS09280 (1789897)	1789897..1790067	-	171	WP_001352098.1	YdaE family protein	-
QMG77_RS09285 (1790067)	1790067..1790288	-	222	WP_000560223.1	killing protein KilR	-
QMG77_RS09290 (1790709)	1790709..1790861	-	153	WP_001427316.1	DUF1391 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	espX7/nleL	1784060..1832756	48696

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-48)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5383.12 Da
• Isoelectric Point: 8.3398

>T43166 WP_001443846.1 NZ_AP027165:c1785987-1785838 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALER

Nucleotide

• Length: 150 bp

>T43166 NZ_AP027165:c1785987-1785838 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAACGATGA

Antitoxin

Nucleotide

• Length: 179 bp

>AT43166 NZ_AP027165:1785616-1785794 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A0D6ZRD3

Antitoxin