Detailed information of TA system TA043153

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4736010..4736612
• Replicon: chromosome
• Accession: NZ_AP027162
• Organism: Escherichia coli strain EH031

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: QMG85_RS24365
• Protein ID: WP_000897305.1
• Coordinates: 4736301..4736612 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: QMG85_RS24360
• Protein ID: WP_000356397.1
• Coordinates: 4736010..4736300 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QMG85_RS24335 (4731556)	4731556..4732191	+	636	WP_000829008.1	formate dehydrogenase cytochrome b556 subunit	-
QMG85_RS24340 (4732188)	4732188..4733117	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
QMG85_RS24345 (4733517)	4733517..4734497	+	981	WP_000399648.1	IS110-like element IS621 family transposase	-
QMG85_RS24350 (4734726)	4734726..4734968	-	243	WP_001086390.1	protein YiiF	-
QMG85_RS24355 (4735187)	4735187..4735405	-	219	WP_001295676.1	CopG family transcriptional regulator	-
QMG85_RS24360 (4736010)	4736010..4736300	-	291	WP_000356397.1	NadS family protein	Antitoxin
QMG85_RS24365 (4736301)	4736301..4736612	-	312	WP_000897305.1	hypothetical protein	Toxin
QMG85_RS24370 (4736841)	4736841..4737749	+	909	WP_001162704.1	alpha/beta hydrolase	-
QMG85_RS24375 (4737813)	4737813..4738754	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
QMG85_RS24380 (4738799)	4738799..4739236	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
QMG85_RS24385 (4739233)	4739233..4740105	-	873	WP_000920762.1	virulence factor BrkB family protein	-
QMG85_RS24390 (4740099)	4740099..4740698	-	600	WP_001315111.1	glucose-1-phosphatase	-
QMG85_RS24395 (4740797)	4740797..4741582	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	4733517..4734497	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T43153 WP_000897305.1 NZ_AP027162:c4736612-4736301 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T43153 NZ_AP027162:c4736612-4736301 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT43153 WP_000356397.1 NZ_AP027162:c4736300-4736010 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT43153 NZ_AP027162:c4736300-4736010 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7