Detailed information of TA system TA043149

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3755984..3756711
• Replicon: chromosome
• Accession: NZ_AP027162
• Organism: Escherichia coli strain EH031

Toxin (Protein)

• Gene name: higB
• Uniprot ID: I2UEW4
• Locus tag: QMG85_RS19600
• Protein ID: WP_000547559.1
• Coordinates: 3756400..3756711 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: QMG85_RS19595
• Protein ID: WP_000126294.1
• Coordinates: 3755984..3756403 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QMG85_RS19575 (3751034)	3751034..3751561	-	528	WP_001078781.1	electron transport protein HydN	-
QMG85_RS19580 (3751710)	3751710..3752720	-	1011	WP_001377591.1	DNA-binding transcriptional regulator AscG	-
QMG85_RS19585 (3752980)	3752980..3754437	+	1458	WP_001107856.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
QMG85_RS19590 (3754446)	3754446..3755870	+	1425	WP_000110313.1	6-phospho-beta-glucosidase AscB	-
QMG85_RS19595 (3755984)	3755984..3756403	-	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
QMG85_RS19600 (3756400)	3756400..3756711	-	312	WP_000547559.1	type II toxin-antitoxin system HigB family toxin	Toxin
QMG85_RS19605 (3756885)	3756885..3757628	-	744	WP_001341829.1	hypothetical protein	-
QMG85_RS19610 (3757671)	3757671..3758141	-	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
QMG85_RS19615 (3758134)	3758134..3758544	-	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
QMG85_RS19620 (3758541)	3758541..3759308	-	768	WP_000067401.1	formate hydrogenlyase subunit HycG	-
QMG85_RS19625 (3759308)	3759308..3759850	-	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
QMG85_RS19630 (3759860)	3759860..3761569	-	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (24-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12450.11 Da
• Isoelectric Point: 9.7249

>T43149 WP_000547559.1 NZ_AP027162:c3756711-3756400 [Escherichia coli]
MHIISKAPFEESARKYPNDALAHQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T43149 NZ_AP027162:c3756711-3756400 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCATCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT43149 WP_000126294.1 NZ_AP027162:c3756403-3755984 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT43149 NZ_AP027162:c3756403-3755984 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCTGTT
GGCGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A0F3V163