Detailed information of TA system TA043114

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4709878..4710480
• Replicon: chromosome
• Accession: NZ_AP027159
• Organism: Escherichia coli strain 2313

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: QMG94_RS24250
• Protein ID: WP_000897305.1
• Coordinates: 4710169..4710480 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: QMG94_RS24245
• Protein ID: WP_000356397.1
• Coordinates: 4709878..4710168 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QMG94_RS24220 (4705424)	4705424..4706059	+	636	WP_000829008.1	formate dehydrogenase cytochrome b556 subunit	-
QMG94_RS24225 (4706056)	4706056..4706985	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
QMG94_RS24230 (4707385)	4707385..4708365	+	981	WP_000399648.1	IS110-like element IS621 family transposase	-
QMG94_RS24235 (4708594)	4708594..4708836	-	243	WP_001086390.1	protein YiiF	-
QMG94_RS24240 (4709055)	4709055..4709273	-	219	WP_001295676.1	CopG family transcriptional regulator	-
QMG94_RS24245 (4709878)	4709878..4710168	-	291	WP_000356397.1	NadS family protein	Antitoxin
QMG94_RS24250 (4710169)	4710169..4710480	-	312	WP_000897305.1	hypothetical protein	Toxin
QMG94_RS24255 (4710709)	4710709..4711617	+	909	WP_001162704.1	alpha/beta hydrolase	-
QMG94_RS24260 (4711681)	4711681..4712622	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
QMG94_RS24265 (4712667)	4712667..4713104	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
QMG94_RS24270 (4713101)	4713101..4713973	-	873	WP_074523435.1	virulence factor BrkB family protein	-
QMG94_RS24275 (4713967)	4713967..4714566	-	600	WP_001315111.1	glucose-1-phosphatase	-
QMG94_RS24280 (4714665)	4714665..4715450	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	4707385..4708365	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T43114 WP_000897305.1 NZ_AP027159:c4710480-4710169 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T43114 NZ_AP027159:c4710480-4710169 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT43114 WP_000356397.1 NZ_AP027159:c4710168-4709878 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT43114 NZ_AP027159:c4710168-4709878 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7