Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4213851..4214650 Replicon chromosome
Accession NZ_AP027159
Organism Escherichia coli strain 2313

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag QMG94_RS21830 Protein ID WP_000347273.1
Coordinates 4214186..4214650 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag QMG94_RS21825 Protein ID WP_001307405.1
Coordinates 4213851..4214186 (+) Length 112 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QMG94_RS21810 (4209636) 4209636..4210406 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
QMG94_RS21815 (4210422) 4210422..4211756 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
QMG94_RS21820 (4212131) 4212131..4213702 + 1572 WP_001273779.1 galactarate dehydratase -
QMG94_RS21825 (4213851) 4213851..4214186 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
QMG94_RS21830 (4214186) 4214186..4214650 + 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
QMG94_RS21835 (4214705) 4214705..4215514 - 810 WP_000072193.1 aga operon transcriptional regulator AgaR -
QMG94_RS21840 (4215763) 4215763..4217043 + 1281 WP_000681914.1 tagatose-bisphosphate aldolase subunit KbaZ -
QMG94_RS21845 (4217066) 4217066..4217539 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
QMG94_RS21850 (4217550) 4217550..4218329 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
QMG94_RS21855 (4218319) 4218319..4219197 + 879 WP_001341890.1 PTS N-acetylgalactosamine transporter subunit IID -
QMG94_RS21860 (4219215) 4219215..4219649 + 435 WP_000948822.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(12-150)

Antitoxin

(14-110)


Sequences


Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T43113 WP_000347273.1 NZ_AP027159:4214186-4214650 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T43113 NZ_AP027159:4214186-4214650 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT43113 WP_001307405.1 NZ_AP027159:4213851-4214186 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT43113 NZ_AP027159:4213851-4214186 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
ACCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTCTTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB V0SSH7


Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References