Detailed information of TA system TA043110

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3715442..3716169
• Replicon: chromosome
• Accession: NZ_AP027159
• Organism: Escherichia coli strain 2313

Toxin (Protein)

• Gene name: higB
• Uniprot ID: I2UEW4
• Locus tag: QMG94_RS19380
• Protein ID: WP_000547559.1
• Coordinates: 3715858..3716169 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: QMG94_RS19375
• Protein ID: WP_000126294.1
• Coordinates: 3715442..3715861 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QMG94_RS19355 (3710492)	3710492..3711019	-	528	WP_001078781.1	electron transport protein HydN	-
QMG94_RS19360 (3711168)	3711168..3712178	-	1011	WP_001377591.1	DNA-binding transcriptional regulator AscG	-
QMG94_RS19365 (3712438)	3712438..3713895	+	1458	WP_001107856.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
QMG94_RS19370 (3713904)	3713904..3715328	+	1425	WP_000110313.1	6-phospho-beta-glucosidase AscB	-
QMG94_RS19375 (3715442)	3715442..3715861	-	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
QMG94_RS19380 (3715858)	3715858..3716169	-	312	WP_000547559.1	type II toxin-antitoxin system HigB family toxin	Toxin
QMG94_RS19385 (3716343)	3716343..3717086	-	744	WP_001341829.1	hypothetical protein	-
QMG94_RS19390 (3717129)	3717129..3717599	-	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
QMG94_RS19395 (3717592)	3717592..3718002	-	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
QMG94_RS19400 (3717999)	3717999..3718766	-	768	WP_000067401.1	formate hydrogenlyase subunit HycG	-
QMG94_RS19405 (3718766)	3718766..3719308	-	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
QMG94_RS19410 (3719318)	3719318..3721027	-	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (24-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12450.11 Da
• Isoelectric Point: 9.7249

>T43110 WP_000547559.1 NZ_AP027159:c3716169-3715858 [Escherichia coli]
MHIISKAPFEESARKYPNDALAHQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T43110 NZ_AP027159:c3716169-3715858 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCATCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT43110 WP_000126294.1 NZ_AP027159:c3715861-3715442 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT43110 NZ_AP027159:c3715861-3715442 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCTGTT
GGCGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A0F3V163