Detailed information of TA system TA043101

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 1759499..1759870
• Replicon: chromosome
• Accession: NZ_AP027159
• Organism: Escherichia coli strain 2313

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A891STD1
• Locus tag: QMG94_RS08970
• Protein ID: WP_042853000.1
• Coordinates: 1759676..1759870 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	1759499..1759677 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QMG94_RS08930 (1754941)	1754941..1755597	-	657	WP_000011658.1	carbon-nitrogen hydrolase family protein YobB	-
QMG94_RS08935 (1755699)	1755699..1755929	-	231	WP_000916763.1	DNA polymerase III subunit theta	-
QMG94_RS08940 (1756068)	1756068..1756442	+	375	WP_000204699.1	CopC domain-containing protein YobA	-
QMG94_RS08945 (1756446)	1756446..1757318	+	873	WP_000879280.1	copper homeostasis membrane protein CopD	-
QMG94_RS08950 (1757331)	1757331..1757672	+	342	WP_000976492.1	YebY family protein	-
QMG94_RS08955 (1758065)	1758065..1759141	-	1077	WP_001189091.1	phage integrase Arm DNA-binding domain-containing protein	-
QMG94_RS08960 (1759107)	1759107..1759388	-	282	WP_001443927.1	excisionase	-
- (1759499)	1759499..1759677	+	179	NuclAT_1	-	Antitoxin
- (1759499)	1759499..1759677	+	179	NuclAT_1	-	Antitoxin
- (1759499)	1759499..1759677	+	179	NuclAT_1	-	Antitoxin
- (1759499)	1759499..1759677	+	179	NuclAT_1	-	Antitoxin
QMG94_RS08965 (1759495)	1759495..1759683	-	189	WP_001356607.1	DUF1187 family protein	-
QMG94_RS08970 (1759676)	1759676..1759870	-	195	WP_042853000.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
QMG94_RS08975 (1759927)	1759927..1760736	-	810	WP_000166317.1	recombination protein RecT	-
QMG94_RS08980 (1760729)	1760729..1763380	-	2652	WP_000105101.1	exodeoxyribonuclease VIII	-
QMG94_RS08985 (1763479)	1763479..1763754	-	276	WP_001307773.1	hypothetical protein	-
QMG94_RS08990 (1763828)	1763828..1763998	-	171	WP_001427414.1	YdaE family protein	-
QMG94_RS08995 (1763998)	1763998..1764219	-	222	WP_000560218.1	killing protein KilR	-
QMG94_RS09000 (1764640)	1764640..1764792	-	153	WP_001427316.1	DUF1391 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleC	1752198..1815333	63135

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7019.87 Da
• Isoelectric Point: 8.6419

>T43101 WP_042853000.1 NZ_AP027159:c1759870-1759676 [Escherichia coli]
MRYDDVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T43101 NZ_AP027159:c1759870-1759676 [Escherichia coli]
ATGAGATATGACGATGTTAAACCATGTCCGTTTTGTGGTTGTCCATCTGTTACAGTGAAAGCAATTTCAGGATATTACCG
GGCAAAATGCAACGGATGCGAATCCCGAACTGGCTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CTACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT43101 NZ_AP027159:1759499-1759677 [Escherichia coli]
GAGGACAGAAGTTTCTCGCAATTAAAATTTATCAGCTTTACTTTCTGCTCTCTGGACACGCCTGCTTCTTTTTTCCCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAATTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCTTTTT
CAATAGTGGCAGTTATTTT

Structures

Antitoxin