Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) hok-sok/-
Location 42604..42868 Replicon plasmid pJNE181771-2
Accession NZ_AP027158
Organism Escherichia coli strain JNE181771

Toxin (Protein)


Gene name pndA Uniprot ID E6BRV3
Locus tag QUD66_RS24815 Protein ID WP_001303307.1
Coordinates 42716..42868 (+) Length 51 a.a.

Antitoxin (RNA)


Gene name pndB
Locus tag -
Coordinates 42604..42661 (-)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUD66_RS24800 (37843) 37843..40134 - 2292 WP_001289276.1 F-type conjugative transfer protein TrbC -
QUD66_RS24805 (40127) 40127..41197 - 1071 WP_000151580.1 IncI1-type conjugal transfer protein TrbB -
QUD66_RS24810 (41216) 41216..42424 - 1209 WP_001303305.1 IncI1-type conjugal transfer protein TrbA -
- (42604) 42604..42661 - 58 NuclAT_0 - Antitoxin
- (42604) 42604..42661 - 58 NuclAT_0 - Antitoxin
- (42604) 42604..42661 - 58 NuclAT_0 - Antitoxin
- (42604) 42604..42661 - 58 NuclAT_0 - Antitoxin
QUD66_RS24815 (42716) 42716..42868 + 153 WP_001303307.1 Hok/Gef family protein Toxin
QUD66_RS24820 (42940) 42940..43191 - 252 WP_001291967.1 hypothetical protein -
QUD66_RS24825 (43690) 43690..43785 + 96 WP_001303310.1 DinQ-like type I toxin DqlB -
QUD66_RS24830 (43850) 43850..44026 - 177 WP_001054898.1 hypothetical protein -
QUD66_RS24835 (44369) 44369..44578 + 210 WP_000062602.1 HEAT repeat domain-containing protein -
QUD66_RS24840 (44650) 44650..45312 - 663 WP_000653333.1 plasmid IncI1-type surface exclusion protein ExcA -
QUD66_RS24845 (45377) 45377..47539 - 2163 WP_000698351.1 DotA/TraY family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..88936 88936


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Hok/gef cell toxic protein

(7-46)


Sequences

Toxin        


Download         Length: 51 a.a.        Molecular weight: 5817.21 Da        Isoelectric Point: 8.7948

>T43081 WP_001303307.1 NZ_AP027158:42716-42868 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCVLRLQQGNTVLVATLAYEVKR

Download         Length: 153 bp

>T43081 NZ_AP027158:42716-42868 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGTACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin


Download         Length: 58 bp

>AT43081 NZ_AP027158:c42661-42604 [Escherichia coli]
GCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A634Q5L1

Antitoxin

Download structure file

References