Detailed information of TA system TA043076

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 104460..104877
• Replicon: plasmid pJNE181771-1
• Accession: NZ_AP027157
• Organism: Escherichia coli strain JNE181771

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: QUD66_RS24345
• Protein ID: WP_223200913.1
• Coordinates: 104460..104582 (-)
• Length: 41 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	104687..104877 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUD66_RS24315	100266..100649	-	384	WP_001151560.1	conjugal transfer relaxosome DNA-binding protein TraM	-
QUD66_RS24320	100934..101581	+	648	WP_135301269.1	transglycosylase SLT domain-containing protein	-
QUD66_RS24325	101877..102698	-	822	WP_052892104.1	DUF932 domain-containing protein	-
QUD66_RS24330	102817..103104	-	288	WP_106914345.1	hypothetical protein	-
QUD66_RS24335	103260..103562	-	303	WP_001272235.1	hypothetical protein	-
QUD66_RS24340	103862..104159	+	298	Protein_107	hypothetical protein	-
QUD66_RS24345	104460..104582	-	123	WP_223200913.1	Hok/Gef family protein	Toxin
QUD66_RS24350	104527..104679	-	153	Protein_109	DUF5431 family protein	-
-	104687..104877	+	191	-	-	Antitoxin
-	104687..104880	-	194	NuclAT_0	-	-
-	104687..104880	-	194	NuclAT_0	-	-
-	104687..104880	-	194	NuclAT_0	-	-
-	104687..104880	-	194	NuclAT_0	-	-
QUD66_RS24355	104829..105143	-	315	WP_000872086.1	hypothetical protein	-
QUD66_RS24360	105140..105859	-	720	WP_001276237.1	plasmid SOS inhibition protein A	-
QUD66_RS24365	105856..106290	-	435	WP_000845923.1	conjugation system SOS inhibitor PsiB	-
QUD66_RS24370	106345..108303	-	1959	WP_052892092.1	ParB/RepB/Spo0J family partition protein	-
QUD66_RS24375	108364..108597	-	234	WP_000005993.1	DUF905 family protein	-
QUD66_RS24380	108655..109176	-	522	WP_032107538.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	hlyD / hlyB / hlyA / hlyC / espP	1..131387	131387

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (1-35)

Sequences

Toxin

Protein

• Length: 41 a.a.
• Molecular weight: 4538.38 Da
• Isoelectric Point: 8.2691

>T43076 WP_223200913.1 NZ_AP027157:c104582-104460 [Escherichia coli]
IVCCTLLIFTLLTRNRLCEVRLKDGYREVTATMAYESGGK

Nucleotide

• Length: 123 bp

>T43076 NZ_AP027157:c104582-104460 [Escherichia coli]
ATCGTGTGCTGCACGTTGTTGATATTCACACTCCTGACCCGGAATCGCCTGTGCGAAGTCCGGCTGAAGGACGGATACAG
GGAAGTTACGGCAACAATGGCTTACGAATCCGGCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 191 bp

>AT43076 NZ_AP027157:104687-104877 [Escherichia coli]
GCCTCGTGGGTTAATGAAAATTAACTACGGGGCTTTCTGCTTTCTGCCACACAACACGGTAACAAACCACCTTCACGTCA
TGAGGCAGAAAGCCTCAAGCGCCGGGCACATCATAGCCCATATACCTGCGCACTGACCACACTCACTTTCCCTGAAAATA
ATCCGGTCGTTCAGCCAGTTCACGGGCAATC

Structures

Antitoxin