Detailed information of TA system TA043073

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 60952..61205
• Replicon: plasmid pJNE181771-1
• Accession: NZ_AP027157
• Organism: Escherichia coli strain JNE181771

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A148HBD8
• Locus tag: QUD66_RS24080
• Protein ID: WP_001336447.1
• Coordinates: 60952..61101 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	61149..61205 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUD66_RS24055 (58037)	58037..58276	-	240	WP_001365702.1	HTH domain-containing protein	-
QUD66_RS24060 (58289)	58289..58549	-	261	WP_072122678.1	hypothetical protein	-
QUD66_RS24065 (59252)	59252..60109	-	858	WP_000130991.1	incFII family plasmid replication initiator RepA	-
QUD66_RS24070 (60102)	60102..60176	-	75	WP_001365705.1	RepA leader peptide Tap	-
QUD66_RS24075 (60411)	60411..60668	-	258	WP_000084404.1	replication regulatory protein RepA	-
QUD66_RS24080 (60952)	60952..61101	-	150	WP_001336447.1	Hok/Gef family protein	Toxin
- (61149)	61149..61205	+	57	NuclAT_1	-	Antitoxin
- (61149)	61149..61205	+	57	NuclAT_1	-	Antitoxin
- (61149)	61149..61205	+	57	NuclAT_1	-	Antitoxin
- (61149)	61149..61205	+	57	NuclAT_1	-	Antitoxin
QUD66_RS24085 (61380)	61380..61763	-	384	WP_001109065.1	hypothetical protein	-
QUD66_RS24090 (61866)	61866..62327	-	462	WP_052892071.1	thermonuclease family protein	-
QUD66_RS24095 (63070)	63070..63273	-	204	WP_001336517.1	hypothetical protein	-
QUD66_RS24100 (63426)	63426..63983	-	558	WP_000139380.1	fertility inhibition protein FinO	-
QUD66_RS24105 (64019)	64019..64387	-	369	WP_000722534.1	NirD/YgiW/YdeI family stress tolerance protein	-
QUD66_RS24110 (64470)	64470..65216	-	747	WP_000205708.1	type-F conjugative transfer system pilin acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	hlyD / hlyB / hlyA / hlyC / espP	1..131387	131387

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5572.69 Da
• Isoelectric Point: 8.7678

>T43073 WP_001336447.1 NZ_AP027157:c61101-60952 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T43073 NZ_AP027157:c61101-60952 [Escherichia coli]
ATGACGAAATATACCCTTATTGGGTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAGCTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 57 bp

>AT43073 NZ_AP027157:61149-61205 [Escherichia coli]
GTACTTCATGCAGGCCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A148HBD8

Antitoxin