Detailed information of TA system TA043069

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4325012..4325614
• Replicon: chromosome
• Accession: NZ_AP027156
• Organism: Escherichia coli strain JNE181771

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: QUD66_RS21125
• Protein ID: WP_000897305.1
• Coordinates: 4325012..4325323 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: QUD66_RS21130
• Protein ID: WP_000356397.1
• Coordinates: 4325324..4325614 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUD66_RS21095 (4320042)	4320042..4320827	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
QUD66_RS21100 (4320926)	4320926..4321525	+	600	WP_001295269.1	glucose-1-phosphatase	-
QUD66_RS21105 (4321519)	4321519..4322391	+	873	WP_000920762.1	virulence factor BrkB family protein	-
QUD66_RS21110 (4322388)	4322388..4322825	+	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
QUD66_RS21115 (4322870)	4322870..4323811	+	942	WP_001343389.1	fatty acid biosynthesis protein FabY	-
QUD66_RS21120 (4323875)	4323875..4324783	-	909	WP_001162704.1	alpha/beta hydrolase	-
QUD66_RS21125 (4325012)	4325012..4325323	+	312	WP_000897305.1	hypothetical protein	Toxin
QUD66_RS21130 (4325324)	4325324..4325614	+	291	WP_000356397.1	NadS family protein	Antitoxin
QUD66_RS21135 (4326219)	4326219..4326437	+	219	WP_001295676.1	CopG family transcriptional regulator	-
QUD66_RS21140 (4326656)	4326656..4326898	+	243	WP_001086388.1	protein YiiF	-
QUD66_RS21145 (4327228)	4327228..4328157	-	930	WP_000027707.1	formate dehydrogenase accessory protein FdhE	-
QUD66_RS21150 (4328154)	4328154..4328789	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
QUD66_RS21155 (4328786)	4328786..4329688	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T43069 WP_000897305.1 NZ_AP027156:4325012-4325323 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T43069 NZ_AP027156:4325012-4325323 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT43069 WP_000356397.1 NZ_AP027156:4325324-4325614 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT43069 NZ_AP027156:4325324-4325614 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7