Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 279886..280427 Replicon chromosome
Accession NZ_AP027156
Organism Escherichia coli strain JNE181771

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag QUD66_RS01265 Protein ID WP_000615976.1
Coordinates 279886..280164 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag QUD66_RS01270 Protein ID WP_000729704.1
Coordinates 280167..280427 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUD66_RS01250 (277468) 277468..278046 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
QUD66_RS01255 (278252) 278252..279019 + 768 WP_000333380.1 class II glutamine amidotransferase -
QUD66_RS01260 (278990) 278990..279730 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
QUD66_RS01265 (279886) 279886..280164 - 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
QUD66_RS01270 (280167) 280167..280427 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
QUD66_RS01275 (280613) 280613..281386 + 774 WP_000543899.1 C40 family peptidase -
QUD66_RS01280 (281443) 281443..281799 - 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
QUD66_RS01285 (281792) 281792..282070 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
QUD66_RS01290 (282175) 282175..283887 - 1713 Protein_250 flagellar biosynthesis protein FlhA -
QUD66_RS01295 (283859) 283859..284644 + 786 WP_000207568.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T43045 WP_000615976.1 NZ_AP027156:c280164-279886 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T43045 NZ_AP027156:c280164-279886 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT43045 WP_000729704.1 NZ_AP027156:c280427-280167 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT43045 NZ_AP027156:c280427-280167 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References