Detailed information of TA system TA042973

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE(toxin)
• Location: 2013287..2013936
• Replicon: chromosome
• Accession: NZ_AP027055
• Organism: Limnohabitans sp. INBF002

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: QMG15_RS10085
• Protein ID: WP_281788499.1
• Coordinates: 2013598..2013936 (-)
• Length: 113 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: QMG15_RS10080
• Protein ID: WP_281788498.1
• Coordinates: 2013287..2013601 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QMG15_RS10055 (LINBF2_19960)	2008401..2009714	-	1314	WP_281790118.1	DEAD/DEAH box helicase	-
QMG15_RS10060 (LINBF2_19970)	2009796..2011382	-	1587	WP_281788495.1	bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase	-
QMG15_RS10065 (LINBF2_19980)	2011395..2011631	-	237	WP_108358066.1	helix-turn-helix domain-containing protein	-
QMG15_RS10070 (LINBF2_19990)	2011624..2012658	-	1035	WP_281788496.1	tRNA dihydrouridine synthase DusB	-
QMG15_RS10075 (LINBF2_20000)	2012792..2013286	+	495	WP_281788497.1	YqaA family protein	-
QMG15_RS10080 (LINBF2_20010)	2013287..2013601	-	315	WP_281788498.1	transcriptional regulator	Antitoxin
QMG15_RS10085 (LINBF2_20020)	2013598..2013936	-	339	WP_281788499.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
QMG15_RS10090 (LINBF2_20030)	2014129..2015670	+	1542	WP_281788500.1	lysine--tRNA ligase	-
QMG15_RS10095 (LINBF2_20040)	2015741..2016127	-	387	WP_108358071.1	hypothetical protein	-
QMG15_RS10100 (LINBF2_20050)	2016132..2017094	-	963	WP_281788501.1	Bug family tripartite tricarboxylate transporter substrate binding protein	-
QMG15_RS10105 (LINBF2_20060)	2017124..2017333	-	210	WP_108358073.1	hypothetical protein	-
QMG15_RS10110 (LINBF2_20070)	2017371..2018453	-	1083	WP_281788502.1	beta-N-acetylhexosaminidase	-
QMG15_RS10115 (LINBF2_20080)	2018470..2018865	-	396	WP_281788503.1	holo-ACP synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (45-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (42-101)

Sequences

Toxin

Protein

• Length: 113 a.a.
• Molecular weight: 13087.96 Da
• Isoelectric Point: 9.3100

>T42973 WP_281788499.1 NZ_AP027055:c2013936-2013598 [Limnohabitans sp. INBF002]
MKAVFVELPAFSRYRTDYLDDEGFRGLQTAIMKNPESGDVIEGTGGLRKLRHADARRGKGKRGGLRVIYYWWDDASQFWL
FTLYDKDEMTDLSPDEKKALKHMLKIELEARR

Nucleotide

• Length: 339 bp

>T42973 NZ_AP027055:c2013936-2013598 [Limnohabitans sp. INBF002]
ATGAAAGCCGTTTTTGTTGAGTTACCAGCGTTTTCGCGCTATCGAACTGACTATCTAGACGATGAAGGTTTTCGTGGGCT
GCAAACGGCCATCATGAAGAACCCTGAATCGGGGGATGTCATTGAAGGTACAGGTGGGCTTCGCAAGTTGCGACACGCTG
ATGCGCGCCGAGGCAAGGGCAAGCGTGGTGGCTTACGAGTCATTTATTACTGGTGGGACGATGCGAGCCAGTTTTGGTTG
TTTACGCTGTACGACAAAGACGAAATGACGGACTTGAGTCCTGATGAAAAGAAGGCACTCAAACACATGTTGAAAATCGA
GCTGGAGGCACGGCGATGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11869.71 Da
• Isoelectric Point: 10.5238

>AT42973 WP_281788498.1 NZ_AP027055:c2013601-2013287 [Limnohabitans sp. INBF002]
MKKRNLFVELSEGFDALGNARAGKQTLRTHVVEDKPAPEVTADELLALRERLHLSRAVFARYLRTKPRTLENWEQGRAKP
NPHAALLIRLVEKYPDTVERLAAV

Nucleotide

• Length: 315 bp

>AT42973 NZ_AP027055:c2013601-2013287 [Limnohabitans sp. INBF002]
ATGAAAAAGCGAAACCTGTTTGTAGAACTGAGCGAAGGCTTTGACGCCCTGGGTAACGCACGTGCGGGTAAACAAACGCT
GCGCACGCATGTGGTGGAGGACAAACCAGCGCCGGAGGTGACAGCCGATGAACTGCTGGCGCTGCGCGAACGCCTGCATT
TGTCGCGCGCTGTGTTTGCGCGTTACTTGCGCACCAAGCCGCGCACACTGGAGAACTGGGAGCAGGGGCGCGCGAAACCT
AATCCACATGCGGCTTTGCTGATTCGCCTCGTCGAAAAGTATCCAGATACGGTTGAGCGATTAGCTGCAGTTTGA