Detailed information of TA system TA042946

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 682534..683189
• Replicon: chromosome
• Accession: NZ_AP026972
• Organism: Polynucleobacter sp. KF022

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: PKF022_RS03615
• Protein ID: WP_281777462.1
• Coordinates: 682854..683189 (-)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: PKF022_RS03610
• Protein ID: WP_281777254.1
• Coordinates: 682534..682848 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PKF022_RS03580 (PKF022_07030)	677715..679040	+	1326	WP_281777249.1	Y-family DNA polymerase	-
PKF022_RS03585	679103..679246	+	144	WP_281777250.1	hypothetical protein	-
PKF022_RS03590 (PKF022_07040)	679407..679949	+	543	WP_068321073.1	TIGR00645 family protein	-
PKF022_RS03595	679979..680329	+	351	WP_281777251.1	YkgJ family cysteine cluster protein	-
PKF022_RS03600 (PKF022_07050)	680322..680981	-	660	WP_281777252.1	DUF3820 family protein	-
PKF022_RS03605 (PKF022_07060)	681318..682427	+	1110	WP_281777253.1	linear amide C-N hydrolase	-
PKF022_RS03610 (PKF022_07070)	682534..682848	-	315	WP_281777254.1	helix-turn-helix transcriptional regulator	Antitoxin
PKF022_RS03615 (PKF022_07080)	682854..683189	-	336	WP_281777462.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PKF022_RS03625 (PKF022_07090)	683631..685301	+	1671	WP_281777255.1	DNA polymerase III subunit gamma/tau	-
PKF022_RS03630 (PKF022_07100)	685369..685692	+	324	WP_072582156.1	YbaB/EbfC family nucleoid-associated protein	-
PKF022_RS03635 (PKF022_07110)	685717..686331	+	615	WP_215349117.1	recombination mediator RecR	-
PKF022_RS03640 (PKF022_07120)	686406..686639	+	234	WP_281777256.1	hypothetical protein	-
PKF022_RS03645 (PKF022_07130)	686636..686908	+	273	WP_281777257.1	type II toxin-antitoxin system RelE/ParE family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	667481..685301	17820

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (18-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (46-99)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 13053.32 Da
• Isoelectric Point: 10.3652

>T42946 WP_281777462.1 NZ_AP026972:c683189-682854 [Polynucleobacter sp. KF022]
ILSIWTIYYFNERTKSEIFSWPVGVLADYRRLLDLMEQYGADLRLPHSRAMGDGLFEIRCRGSEGIGRVFYGMMIGKRII
VLHSFIKKTQETPDKEIKKARKRLKEVKERG

Nucleotide

• Length: 336 bp

>T42946 NZ_AP026972:c683189-682854 [Polynucleobacter sp. KF022]
ATCTTGAGCATTTGGACTATCTACTACTTTAATGAGCGCACTAAATCTGAGATTTTTTCTTGGCCTGTAGGAGTTTTGGC
GGATTACAGGCGCCTGCTAGATTTGATGGAGCAATACGGTGCTGATCTAAGACTCCCCCACTCTAGGGCAATGGGTGATG
GCTTATTTGAAATACGTTGTAGGGGTAGCGAAGGTATTGGGCGAGTTTTCTACGGGATGATGATTGGTAAACGCATCATT
GTTTTGCATAGCTTTATTAAGAAAACGCAAGAGACCCCAGATAAGGAAATAAAGAAGGCGCGCAAGCGACTAAAAGAGGT
GAAAGAAAGAGGTTAA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11813.55 Da
• Isoelectric Point: 10.0090

>AT42946 WP_281777254.1 NZ_AP026972:c682848-682534 [Polynucleobacter sp. KF022]
MIKKKDRYAPVPHSRQDDEKLFANPQFKKAWDALEEEYAALSELFQARKEAGLTQEQVAQIMGTTKSAISRLESSIRSDS
HSPSFSTLRKYAHACGKKLLIKMV

Nucleotide

• Length: 315 bp

>AT42946 NZ_AP026972:c682848-682534 [Polynucleobacter sp. KF022]
ATGATTAAGAAAAAAGATCGATATGCACCTGTACCCCACTCTCGCCAAGACGATGAAAAGTTATTTGCTAACCCGCAGTT
TAAAAAGGCTTGGGATGCTCTAGAAGAAGAATATGCAGCTCTTTCTGAATTATTTCAGGCCCGCAAAGAAGCAGGGCTTA
CTCAAGAACAGGTTGCGCAAATTATGGGAACAACAAAAAGCGCTATCTCACGCCTCGAATCATCCATTCGGAGCGATAGC
CATTCACCATCGTTTTCAACCTTACGAAAGTACGCTCATGCCTGCGGAAAGAAGCTTCTGATCAAAATGGTCTAG