Detailed information of TA system TA042945

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-YefM
• Location: 288356..288858
• Replicon: chromosome
• Accession: NZ_AP026972
• Organism: Polynucleobacter sp. KF022

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: PKF022_RS01575
• Protein ID: WP_281776940.1
• Coordinates: 288595..288858 (+)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: PKF022_RS01570
• Protein ID: WP_215360829.1
• Coordinates: 288356..288598 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PKF022_RS01540 (PKF022_02930)	283506..284270	-	765	WP_281776934.1	3-deoxy-manno-octulosonate cytidylyltransferase	-
PKF022_RS01545 (PKF022_02940)	284292..284471	-	180	WP_281776935.1	Trm112 family protein	-
PKF022_RS01550 (PKF022_02950)	284544..285635	-	1092	WP_281776936.1	tetraacyldisaccharide 4'-kinase	-
PKF022_RS01555 (PKF022_02960)	285660..286127	-	468	WP_281776937.1	biopolymer transporter ExbD	-
PKF022_RS01560 (PKF022_02970)	286124..286771	-	648	WP_281776938.1	MotA/TolQ/ExbB proton channel family protein	-
PKF022_RS01565 (PKF022_02980)	287085..288296	+	1212	WP_281776939.1	exodeoxyribonuclease VII large subunit	-
PKF022_RS01570 (PKF022_02990)	288356..288598	+	243	WP_215360829.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
PKF022_RS01575 (PKF022_03000)	288595..288858	+	264	WP_281776940.1	Txe/YoeB family addiction module toxin	Toxin
PKF022_RS01580 (PKF022_03010)	288863..289900	-	1038	WP_281776941.1	UDP-N-acetylmuramate dehydrogenase	-
PKF022_RS01585 (PKF022_03020)	290032..290517	+	486	WP_281776942.1	YajQ family cyclic di-GMP-binding protein	-
PKF022_RS01590 (PKF022_03030)	290510..291436	+	927	WP_281776943.1	site-specific tyrosine recombinase XerD	-
PKF022_RS01595 (PKF022_03040)	291496..292128	+	633	WP_281776944.1	glycerol-3-phosphate 1-O-acyltransferase PlsY	-
PKF022_RS01600 (PKF022_03050)	292150..292521	+	372	WP_281776945.1	MAPEG family protein	-
PKF022_RS01605 (PKF022_03060)	292588..293352	+	765	WP_281776946.1	GTP cyclohydrolase I	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (23-86)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-69)

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10203.98 Da
• Isoelectric Point: 10.4619

>T42945 WP_281776940.1 NZ_AP026972:288595-288858 [Polynucleobacter sp. KF022]
VTWNLVFSKYAIKDAKKLSAAGLKEKAQSLLKVLEADPLQNPPPYEKLVGDLKGAYSRRINIQHRMVYEVFRKEKTVRIL
RMWTHYD

Nucleotide

• Length: 264 bp

>T42945 NZ_AP026972:288595-288858 [Polynucleobacter sp. KF022]
GTGACTTGGAATTTAGTATTTTCCAAGTATGCAATAAAAGATGCCAAAAAATTATCTGCCGCTGGATTAAAAGAAAAAGC
ACAGTCTTTGCTTAAAGTATTAGAAGCAGATCCACTGCAAAATCCACCGCCTTACGAGAAATTAGTGGGAGATTTAAAGG
GCGCGTATTCTCGAAGAATTAATATTCAACATCGCATGGTTTATGAAGTTTTTCGTAAAGAAAAAACAGTTCGAATATTG
AGAATGTGGACTCACTACGATTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8876.28 Da
• Isoelectric Point: 8.5552

>AT42945 WP_215360829.1 NZ_AP026972:288356-288598 [Polynucleobacter sp. KF022]
MTTITASEARAGLYRLIDQAAESHKPVTISGKRANAVLISEEDWSAIQETLYLMAIPGMRESIKDAMAEPLSKSKRVLKW

Nucleotide

• Length: 243 bp

>AT42945 NZ_AP026972:288356-288598 [Polynucleobacter sp. KF022]
ATGACAACTATTACCGCAAGTGAAGCTAGAGCAGGGCTTTATCGCTTGATTGATCAGGCGGCAGAGTCTCATAAGCCTGT
GACGATTTCCGGAAAGCGAGCTAACGCTGTGCTGATTTCTGAGGAGGACTGGAGTGCAATTCAGGAGACGCTATATCTTA
TGGCGATACCTGGAATGCGTGAGTCTATTAAAGATGCGATGGCAGAGCCCTTATCAAAGAGTAAAAGGGTATTAAAGTGG
TGA