42942

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4134399..4134896 Replicon chromosome
Accession NZ_AP026961
Organism Escherichia coli strain EC20-72C-1

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag OO975_RS20150 Protein ID WP_001173343.1
Coordinates 4134399..4134572 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag OO975_RS20155 Protein ID WP_000398619.1
Coordinates 4134600..4134896 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OO975_RS20140 (4131069) 4131069..4132526 + 1458 WP_000856841.1 dipeptide/tripeptide permease DtpC -
OO975_RS20145 (4132763) 4132763..4134280 + 1518 WP_001295074.1 lysine--tRNA ligase -
OO975_RS20150 (4134399) 4134399..4134572 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
OO975_RS20155 (4134600) 4134600..4134896 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
OO975_RS20160 (4135123) 4135123..4135395 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
OO975_RS20165 (4135407) 4135407..4135637 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
OO975_RS20170 (4135818) 4135818..4137449 + 1632 WP_001565376.1 sensor histidine kinase -
OO975_RS20175 (4137446) 4137446..4138165 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 4125545..4134896 9351


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T42942 WP_001173343.1 NZ_AP026961:c4134572-4134399 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T42942 NZ_AP026961:c4134572-4134399 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT42942 WP_000398619.1 NZ_AP026961:c4134896-4134600 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT42942 NZ_AP026961:c4134896-4134600 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBH6

References