Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3610476..3611017 Replicon chromosome
Accession NZ_AP026961
Organism Escherichia coli strain EC20-72C-1

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag OO975_RS17625 Protein ID WP_000615976.1
Coordinates 3610739..3611017 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag OO975_RS17620 Protein ID WP_000729704.1
Coordinates 3610476..3610736 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OO975_RS17600 (3606151) 3606151..3606936 - 786 WP_000207587.1 putative lateral flagellar export/assembly protein LafU -
OO975_RS17605 (3606908) 3606908..3608620 + 1713 Protein_3446 flagellar biosynthesis protein FlhA -
OO975_RS17610 (3608844) 3608844..3609341 - 498 WP_089615104.1 REP-associated tyrosine transposase RayT -
OO975_RS17615 (3609517) 3609517..3610290 - 774 WP_015675129.1 C40 family peptidase -
OO975_RS17620 (3610476) 3610476..3610736 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
OO975_RS17625 (3610739) 3610739..3611017 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
OO975_RS17630 (3611173) 3611173..3611913 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
OO975_RS17635 (3611884) 3611884..3612651 - 768 WP_000333379.1 class II glutamine amidotransferase -
OO975_RS17640 (3612857) 3612857..3613435 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 3603318..3620495 17177


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T42940 WP_000615976.1 NZ_AP026961:3610739-3611017 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T42940 NZ_AP026961:3610739-3611017 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT42940 WP_000729704.1 NZ_AP026961:3610476-3610736 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT42940 NZ_AP026961:3610476-3610736 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References