Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | yafN-yafO (relBE)/YafO-YafN |
| Location | 3604279..3604973 | Replicon | chromosome |
| Accession | NZ_AP026961 | ||
| Organism | Escherichia coli strain EC20-72C-1 | ||
Toxin (Protein)
| Gene name | yafO | Uniprot ID | S1EXB8 |
| Locus tag | OO975_RS17585 | Protein ID | WP_001263493.1 |
| Coordinates | 3604279..3604677 (-) | Length | 133 a.a. |
Antitoxin (Protein)
| Gene name | yafN | Uniprot ID | S1FJN6 |
| Locus tag | OO975_RS17590 | Protein ID | WP_000554757.1 |
| Coordinates | 3604680..3604973 (-) | Length | 98 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| - (3599940) | 3599940..3600020 | - | 81 | NuclAT_9 | - | - |
| - (3599940) | 3599940..3600020 | - | 81 | NuclAT_9 | - | - |
| - (3599940) | 3599940..3600020 | - | 81 | NuclAT_9 | - | - |
| - (3599940) | 3599940..3600020 | - | 81 | NuclAT_9 | - | - |
| OO975_RS17555 (3599280) | 3599280..3600524 | - | 1245 | WP_000189541.1 | esterase FrsA | - |
| OO975_RS17560 (3600616) | 3600616..3601074 | - | 459 | WP_001291990.1 | xanthine phosphoribosyltransferase | - |
| OO975_RS17565 (3601335) | 3601335..3602792 | + | 1458 | WP_001293003.1 | cytosol nonspecific dipeptidase | - |
| OO975_RS17570 (3602849) | 3602849..3603370 | - | 522 | Protein_3439 | peptide chain release factor H | - |
| OO975_RS17575 (3603369) | 3603369..3603572 | - | 204 | Protein_3440 | RtcB family protein | - |
| OO975_RS17580 (3603817) | 3603817..3604269 | - | 453 | WP_001059847.1 | GNAT family N-acetyltransferase | - |
| OO975_RS17585 (3604279) | 3604279..3604677 | - | 399 | WP_001263493.1 | type II toxin-antitoxin system mRNA interferase toxin YafO | Toxin |
| OO975_RS17590 (3604680) | 3604680..3604973 | - | 294 | WP_000554757.1 | type I toxin-antitoxin system antitoxin YafN | Antitoxin |
| OO975_RS17595 (3605025) | 3605025..3606080 | - | 1056 | WP_001226164.1 | DNA polymerase IV | - |
| OO975_RS17600 (3606151) | 3606151..3606936 | - | 786 | WP_000207587.1 | putative lateral flagellar export/assembly protein LafU | - |
| OO975_RS17605 (3606908) | 3606908..3608620 | + | 1713 | Protein_3446 | flagellar biosynthesis protein FlhA | - |
| OO975_RS17610 (3608844) | 3608844..3609341 | - | 498 | WP_089615104.1 | REP-associated tyrosine transposase RayT | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Genomic island | - | gmhA/lpcA | 3603318..3620495 | 17177 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 133 a.a. Molecular weight: 15472.83 Da Isoelectric Point: 8.0949
>T42939 WP_001263493.1 NZ_AP026961:c3604677-3604279 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF
Download Length: 399 bp
>T42939 NZ_AP026961:c3604677-3604279 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA
Antitoxin
Download Length: 98 a.a. Molecular weight: 11233.81 Da Isoelectric Point: 5.9050
>AT42939 WP_000554757.1 NZ_AP026961:c3604973-3604680 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE
Download Length: 294 bp
>AT42939 NZ_AP026961:c3604973-3604680 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|