Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1719558..1720060 Replicon chromosome
Accession NZ_AP026961
Organism Escherichia coli strain EC20-72C-1

Toxin (Protein)


Gene name yoeB Uniprot ID A0A8E0FJZ4
Locus tag OO975_RS08130 Protein ID WP_000767833.1
Coordinates 1719806..1720060 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag OO975_RS08125 Protein ID WP_001259255.1
Coordinates 1719558..1719809 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OO975_RS08100 (1714737) 1714737..1715804 - 1068 WP_032202492.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
OO975_RS08105 (1715804) 1715804..1716874 - 1071 WP_000108970.1 histidinol-phosphate transaminase -
OO975_RS08110 (1716871) 1716871..1718175 - 1305 WP_058905757.1 histidinol dehydrogenase -
OO975_RS08115 (1718181) 1718181..1719080 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
OO975_RS08120 (1719226) 1719226..1719276 - 51 WP_001364200.1 his operon leader peptide -
OO975_RS08125 (1719558) 1719558..1719809 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
OO975_RS08130 (1719806) 1719806..1720060 + 255 WP_000767833.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
OO975_RS08135 (1720143) 1720143..1720967 + 825 WP_032202495.1 SDR family oxidoreductase -
OO975_RS08140 (1721013) 1721013..1721942 + 930 WP_000803351.1 LysR substrate-binding domain-containing protein -
OO975_RS08145 (1722157) 1722157..1722219 + 63 WP_010723108.1 membrane protein YoeI -
OO975_RS08150 (1722209) 1722209..1723567 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
OO975_RS08155 (1723746) 1723746..1724804 + 1059 WP_000492312.1 thiosulfate utilization transporter TsuA/YeeE -
OO975_RS08160 (1724818) 1724818..1725045 + 228 WP_000985278.1 thiosulfate utilization sulfurtransferase TsuB/YeeD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10173.54 Da        Isoelectric Point: 8.0353

>T42926 WP_000767833.1 NZ_AP026961:1719806-1720060 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPESLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T42926 NZ_AP026961:1719806-1720060 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAATCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT42926 WP_001259255.1 NZ_AP026961:1719558-1719809 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT42926 NZ_AP026961:1719558-1719809 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References