Detailed information of TA system TA042922

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 600898..601691
• Replicon: chromosome
• Accession: NZ_AP026961
• Organism: Escherichia coli strain EC20-72C-1

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: -
• Locus tag: OO975_RS02915
• Protein ID: WP_112921698.1
• Coordinates: 600898..601356 (-)
• Length: 153 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1EB98
• Locus tag: OO975_RS02920
• Protein ID: WP_001307405.1
• Coordinates: 601356..601691 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OO975_RS02890 (597492)	597492..598187	-	696	WP_000044775.1	molecular chaperone	-
OO975_RS02895 (598267)	598267..598851	-	585	WP_001045432.1	fimbrial protein	-
OO975_RS02900 (599252)	599252..600007	-	756	WP_001300536.1	galactosamine-6-phosphate isomerase	-
OO975_RS02905 (600008)	600008..600151	-	144	Protein_569	PTS system mannose/fructose/sorbose family transporter subunit IID	-
OO975_RS02910 (600207)	600207..600904	+	698	WP_094096600.1	IS1-like element IS1A family transposase	-
OO975_RS02915 (600898)	600898..601356	-	459	WP_112921698.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
OO975_RS02920 (601356)	601356..601691	-	336	WP_001307405.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
OO975_RS02925 (601840)	601840..603411	-	1572	WP_112921699.1	galactarate dehydratase	-
OO975_RS02930 (603786)	603786..605120	+	1335	WP_001723935.1	galactarate/glucarate/glycerate transporter GarP	-
OO975_RS02935 (605136)	605136..605906	+	771	WP_001723934.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	597492..612451	14959

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-148)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 153 a.a.
• Molecular weight: 17545.09 Da
• Isoelectric Point: 9.9309

>T42922 WP_112921698.1 NZ_AP026961:c601356-600898 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLVMTPTY

Nucleotide

• Length: 459 bp

>T42922 NZ_AP026961:c601356-600898 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTGGTAATGACTCCAACTTATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12358.94 Da
• Isoelectric Point: 4.8616

>AT42922 WP_001307405.1 NZ_AP026961:c601691-601356 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT42922 NZ_AP026961:c601691-601356 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0XVC7