Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | higBA (relBE)/COG4683-HTH_37 |
| Location | 14956..15613 | Replicon | plasmid pEC20-4B-2-2 |
| Accession | NZ_AP026939 | ||
| Organism | Escherichia coli strain EC20-4B-2 | ||
Toxin (Protein)
| Gene name | higB | Uniprot ID | U3PDC3 |
| Locus tag | OO845_RS23305 | Protein ID | WP_000270043.1 |
| Coordinates | 15263..15613 (-) | Length | 117 a.a. |
Antitoxin (Protein)
| Gene name | higA | Uniprot ID | - |
| Locus tag | OO845_RS23300 | Protein ID | WP_000124640.1 |
| Coordinates | 14956..15258 (-) | Length | 101 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| OO845_RS23255 (EC204B2_44610) | 10581..11009 | + | 429 | WP_000591074.1 | hypothetical protein | - |
| OO845_RS23260 (EC204B2_44620) | 11066..11425 | + | 360 | WP_000422768.1 | hypothetical protein | - |
| OO845_RS23265 (EC204B2_44630) | 11425..11871 | + | 447 | WP_000919345.1 | Fe3+-siderophore ABC transporter permease | - |
| OO845_RS23270 (EC204B2_44640) | 11868..12386 | + | 519 | WP_000210756.1 | nitrite reductase | - |
| OO845_RS23275 (EC204B2_44650) | 12386..12616 | + | 231 | WP_000972663.1 | hypothetical protein | - |
| OO845_RS23280 (EC204B2_44660) | 12603..13460 | + | 858 | WP_001167032.1 | hypothetical protein | - |
| OO845_RS23285 (EC204B2_44680) | 13691..14218 | + | 528 | WP_001236377.1 | thermonuclease family protein | - |
| OO845_RS23290 (EC204B2_44690) | 14276..14548 | + | 273 | WP_001043047.1 | HU family DNA-binding protein | - |
| OO845_RS23295 (EC204B2_44700) | 14636..14929 | + | 294 | WP_001239997.1 | chromosome segregation protein ParM | - |
| OO845_RS23300 (EC204B2_44710) | 14956..15258 | - | 303 | WP_000124640.1 | XRE family transcriptional regulator | Antitoxin |
| OO845_RS23305 (EC204B2_44720) | 15263..15613 | - | 351 | WP_000270043.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| OO845_RS23310 (EC204B2_44730) | 15776..16324 | + | 549 | WP_001061574.1 | transcriptional regulator | - |
| OO845_RS23315 | 16546..17714 | + | 1169 | WP_108454466.1 | IS3-like element ISEc52 family transposase | - |
| OO845_RS23320 (EC204B2_44760) | 17917..18111 | + | 195 | WP_000343597.1 | hypothetical protein | - |
| OO845_RS23325 (EC204B2_44770) | 18122..18493 | + | 372 | WP_000516916.1 | hypothetical protein | - |
| OO845_RS23330 (EC204B2_44780) | 18486..18956 | + | 471 | WP_001281821.1 | hypothetical protein | - |
| OO845_RS23335 (EC204B2_44790) | 18971..19306 | - | 336 | WP_000683476.1 | hypothetical protein | - |
| OO845_RS23340 (EC204B2_44800) | 19403..19888 | + | 486 | WP_211120005.1 | DUF1643 domain-containing protein | - |
| OO845_RS23345 (EC204B2_44810) | 20113..20550 | - | 438 | Protein_32 | transposase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | sul1 / aph(3')-VI / blaNDM-1 | - | 1..137080 | 137080 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 117 a.a. Molecular weight: 13328.21 Da Isoelectric Point: 5.2421
>T42902 WP_000270043.1 NZ_AP026939:c15613-15263 [Escherichia coli]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE
Download Length: 351 bp
>T42902 NZ_AP026939:c15613-15263 [Escherichia coli]
ATGTGGGTCATCGAGACAACCGACACCTTTGATGAGTGGTTCGATGCTCTGGATGATACCGATAGAGCAAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGCCCCATGCTGTCGAGGCCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTTCGGGTCCAAAGCAAAGGAGATCCTATCAGAGCGTTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGTGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA
ATGTGGGTCATCGAGACAACCGACACCTTTGATGAGTGGTTCGATGCTCTGGATGATACCGATAGAGCAAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGCCCCATGCTGTCGAGGCCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTTCGGGTCCAAAGCAAAGGAGATCCTATCAGAGCGTTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGTGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA
Antitoxin
Download Length: 101 a.a. Molecular weight: 11006.73 Da Isoelectric Point: 7.2036
>AT42902 WP_000124640.1 NZ_AP026939:c15258-14956 [Escherichia coli]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV
Download Length: 303 bp
>AT42902 NZ_AP026939:c15258-14956 [Escherichia coli]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|