Detailed information of TA system TA042894

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 2694584..2695186
• Replicon: chromosome
• Accession: NZ_AP026937
• Organism: Escherichia coli strain EC20-4B-2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: OO845_RS13025
• Protein ID: WP_000897305.1
• Coordinates: 2694875..2695186 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: OO845_RS13020
• Protein ID: WP_000356397.1
• Coordinates: 2694584..2694874 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OO845_RS12995 (2690509)	2690509..2691411	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
OO845_RS13000 (2691408)	2691408..2692043	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
OO845_RS13005 (2692040)	2692040..2692969	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
OO845_RS13010 (2693299)	2693299..2693541	-	243	WP_001087409.1	protein YiiF	-
OO845_RS13015 (2693761)	2693761..2693979	-	219	WP_001315930.1	CopG family transcriptional regulator	-
OO845_RS13020 (2694584)	2694584..2694874	-	291	WP_000356397.1	NadS family protein	Antitoxin
OO845_RS13025 (2694875)	2694875..2695186	-	312	WP_000897305.1	hypothetical protein	Toxin
OO845_RS13030 (2695415)	2695415..2696323	+	909	Protein_2542	alpha/beta hydrolase	-
OO845_RS13035 (2696387)	2696387..2697328	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
OO845_RS13040 (2697373)	2697373..2697810	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
OO845_RS13045 (2697807)	2697807..2698679	-	873	WP_000920762.1	virulence factor BrkB family protein	-
OO845_RS13050 (2698673)	2698673..2699272	-	600	WP_001295269.1	glucose-1-phosphatase	-
OO845_RS13055 (2699371)	2699371..2700156	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T42894 WP_000897305.1 NZ_AP026937:c2695186-2694875 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T42894 NZ_AP026937:c2695186-2694875 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTATTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT42894 WP_000356397.1 NZ_AP026937:c2694874-2694584 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT42894 NZ_AP026937:c2694874-2694584 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7