Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 1878097..1878638 Replicon chromosome
Accession NZ_AP026937
Organism Escherichia coli strain EC20-4B-2

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag OO845_RS09265 Protein ID WP_000615976.1
Coordinates 1878360..1878638 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag OO845_RS09260 Protein ID WP_000729704.1
Coordinates 1878097..1878357 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OO845_RS09235 (1873880) 1873880..1874665 - 786 WP_264832224.1 putative lateral flagellar export/assembly protein LafU -
OO845_RS09240 (1874637) 1874637..1876349 + 1713 Protein_1811 flagellar biosynthesis protein FlhA -
OO845_RS09245 (1876454) 1876454..1876732 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
OO845_RS09250 (1876725) 1876725..1877081 + 357 WP_001030483.1 type II toxin-antitoxin system HicB family antitoxin -
OO845_RS09255 (1877138) 1877138..1877911 - 774 WP_000543895.1 C40 family peptidase -
OO845_RS09260 (1878097) 1878097..1878357 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
OO845_RS09265 (1878360) 1878360..1878638 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
OO845_RS09270 (1878794) 1878794..1879534 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
OO845_RS09275 (1879505) 1879505..1880272 - 768 WP_180397265.1 class II glutamine amidotransferase -
OO845_RS09280 (1880478) 1880478..1881056 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA / rhs/PAAR / tssM / hcp1/tssD1 1876725..1903378 26653


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T42891 WP_000615976.1 NZ_AP026937:1878360-1878638 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T42891 NZ_AP026937:1878360-1878638 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT42891 WP_000729704.1 NZ_AP026937:1878097-1878357 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT42891 NZ_AP026937:1878097-1878357 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References