Detailed information of TA system TA042869

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 3811308..3811905
• Replicon: chromosome
• Accession: NZ_AP026913
• Organism: Klebsiella pneumoniae strain Kp21r_f22

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A9J6S5A1
• Locus tag: kp21f22_RS18835
• Protein ID: WP_004893639.1
• Coordinates: 3811588..3811905 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: R4YH91
• Locus tag: kp21f22_RS18830
• Protein ID: WP_004142561.1
• Coordinates: 3811308..3811595 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
kp21f22_RS18800 (3807525)	3807525..3807773	+	249	WP_002893037.1	DUF1158 domain-containing protein	-
kp21f22_RS18805 (3807791)	3807791..3808132	-	342	WP_002893035.1	RamA family antibiotic efflux transcriptional regulator	-
kp21f22_RS18810 (3808163)	3808163..3809278	-	1116	Protein_3686	MBL fold metallo-hydrolase	-
kp21f22_RS18815 (3809458)	3809458..3810029	+	572	Protein_3687	TetR/AcrR family transcriptional regulator	-
kp21f22_RS18820 (3810029)	3810029..3810397	+	369	WP_004142557.1	MmcQ/YjbR family DNA-binding protein	-
kp21f22_RS18825 (3810517)	3810517..3811170	+	654	WP_004178896.1	oxygen-insensitive NAD(P)H nitroreductase	-
kp21f22_RS18830 (3811308)	3811308..3811595	-	288	WP_004142561.1	helix-turn-helix transcriptional regulator	Antitoxin
kp21f22_RS18835 (3811588)	3811588..3811905	-	318	WP_004893639.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
kp21f22_RS18840 (3812090)	3812090..3813133	-	1044	WP_023279204.1	DUF2157 domain-containing protein	-
kp21f22_RS18845 (3813803)	3813803..3814669	-	867	WP_004151823.1	helix-turn-helix transcriptional regulator	-
kp21f22_RS18850 (3814778)	3814778..3816205	+	1428	WP_004176980.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-88)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12100.35 Da
• Isoelectric Point: 11.2767

>T42869 WP_004893639.1 NZ_AP026913:c3811905-3811588 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPSIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T42869 NZ_AP026913:c3811905-3811588 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGTCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10771.46 Da
• Isoelectric Point: 10.2212

>AT42869 WP_004142561.1 NZ_AP026913:c3811595-3811308 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Nucleotide

• Length: 288 bp

>AT42869 NZ_AP026913:c3811595-3811308 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACGTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Structures

Antitoxin

• AlphaFold DB: A0A0S3DIQ1