Detailed information of TA system TA042830

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4770344..4770946
• Replicon: chromosome
• Accession: NZ_AP026907
• Organism: Escherichia coli strain MY732

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: MY015_RS23170
• Protein ID: WP_000897305.1
• Coordinates: 4770635..4770946 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MY015_RS23165
• Protein ID: WP_000356397.1
• Coordinates: 4770344..4770634 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MY015_RS23140 (4766269)	4766269..4767171	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
MY015_RS23145 (4767168)	4767168..4767803	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
MY015_RS23150 (4767800)	4767800..4768729	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
MY015_RS23155 (4769059)	4769059..4769301	-	243	WP_001087409.1	protein YiiF	-
MY015_RS23160 (4769521)	4769521..4769739	-	219	WP_001315930.1	CopG family transcriptional regulator	-
MY015_RS23165 (4770344)	4770344..4770634	-	291	WP_000356397.1	NadS family protein	Antitoxin
MY015_RS23170 (4770635)	4770635..4770946	-	312	WP_000897305.1	hypothetical protein	Toxin
MY015_RS23175 (4771175)	4771175..4772083	+	909	WP_001162704.1	alpha/beta hydrolase	-
MY015_RS23180 (4772147)	4772147..4773088	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
MY015_RS23185 (4773133)	4773133..4773570	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
MY015_RS23190 (4773567)	4773567..4774439	-	873	WP_000920762.1	virulence factor BrkB family protein	-
MY015_RS23195 (4774433)	4774433..4775032	-	600	WP_001295269.1	glucose-1-phosphatase	-
MY015_RS23200 (4775131)	4775131..4775916	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T42830 WP_000897305.1 NZ_AP026907:c4770946-4770635 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T42830 NZ_AP026907:c4770946-4770635 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTATTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT42830 WP_000356397.1 NZ_AP026907:c4770634-4770344 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT42830 NZ_AP026907:c4770634-4770344 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7