Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3854423..3854964 Replicon chromosome
Accession NZ_AP026907
Organism Escherichia coli strain MY732

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag MY015_RS18845 Protein ID WP_000615976.1
Coordinates 3854686..3854964 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag MY015_RS18840 Protein ID WP_000729704.1
Coordinates 3854423..3854683 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MY015_RS18815 (3849459) 3849459..3850598 + 1140 WP_000785662.1 flagellar type III secretion system protein FlhB -
MY015_RS18820 (3850582) 3850582..3852675 + 2094 WP_001597626.1 flagellar biosynthesis protein FlhA -
MY015_RS18825 (3852780) 3852780..3853058 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
MY015_RS18830 (3853051) 3853051..3853407 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
MY015_RS18835 (3853464) 3853464..3854237 - 774 WP_000543897.1 C40 family peptidase -
MY015_RS18840 (3854423) 3854423..3854683 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
MY015_RS18845 (3854686) 3854686..3854964 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
MY015_RS18850 (3855120) 3855120..3855860 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
MY015_RS18855 (3855831) 3855831..3856598 - 768 WP_000333380.1 class II glutamine amidotransferase -
MY015_RS18860 (3856804) 3856804..3857382 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T42826 WP_000615976.1 NZ_AP026907:3854686-3854964 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T42826 NZ_AP026907:3854686-3854964 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT42826 WP_000729704.1 NZ_AP026907:3854423-3854683 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT42826 NZ_AP026907:3854423-3854683 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References