Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3842210..3842867 Replicon chromosome
Accession NZ_AP026886
Organism Enterobacter asburiae strain KAM576

Toxin (Protein)


Gene name higB Uniprot ID A0A808IF03
Locus tag OO861_RS18735 Protein ID WP_033146338.1
Coordinates 3842210..3842509 (+) Length 100 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A808ICP3
Locus tag OO861_RS18740 Protein ID WP_033146339.1
Coordinates 3842520..3842867 (+) Length 116 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OO861_RS18725 (KAM576c_36720) 3838580..3840778 + 2199 WP_182382597.1 type I secretion system permease/ATPase -
OO861_RS18730 (KAM576c_36730) 3840789..3841958 + 1170 WP_086374946.1 HlyD family efflux transporter periplasmic adaptor subunit -
OO861_RS18735 3842210..3842509 + 300 WP_033146338.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
OO861_RS18740 (KAM576c_36740) 3842520..3842867 + 348 WP_033146339.1 HigA family addiction module antitoxin Antitoxin
OO861_RS18745 (KAM576c_36750) 3842912..3843337 + 426 WP_064673552.1 nucleoside triphosphatase NudI -
OO861_RS18750 (KAM576c_36760) 3843309..3844040 - 732 WP_264884742.1 helix-turn-helix transcriptional regulator -
OO861_RS18755 (KAM576c_36780) 3844138..3844557 + 420 WP_039263434.1 nuclear transport factor 2 family protein -
OO861_RS18760 (KAM576c_36790) 3844560..3845078 - 519 WP_069303206.1 hypothetical protein -
OO861_RS18765 (KAM576c_36800) 3845163..3846344 - 1182 WP_182382598.1 PLP-dependent aminotransferase family protein -
OO861_RS18770 (KAM576c_36810) 3846364..3847251 - 888 WP_193139843.1 LysR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 3783648..3857617 73969


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(1-95)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(8-86)


Sequences

Toxin        


Download         Length: 100 a.a.        Molecular weight: 11691.32 Da        Isoelectric Point: 10.0346

>T42802 WP_033146338.1 NZ_AP026886:3842210-3842509 [Enterobacter asburiae]
MISYFRDQWLEDFFLYGRSSNVIPANLETALARKLDIIRAATSHRDLRSPPGNMYEALNPPLKGYSSIRVNRQYRLVFRW
TEGKAEDLYLSPHKYTQHK

Download         Length: 300 bp

>T42802 NZ_AP026886:3842210-3842509 [Enterobacter asburiae]
ATGATTAGTTATTTTCGGGATCAATGGCTTGAGGATTTTTTTCTTTACGGCAGATCGAGCAATGTTATTCCTGCAAATCT
GGAAACAGCGCTTGCGAGAAAGCTCGATATCATCAGGGCAGCAACCTCGCACAGGGATTTGCGATCGCCACCGGGCAATA
TGTATGAAGCATTGAATCCTCCGCTGAAGGGATATTCCTCTATCCGGGTAAACAGGCAATACAGGCTCGTATTTCGCTGG
ACAGAGGGTAAAGCAGAAGATCTCTACCTCTCTCCACACAAGTACACGCAACACAAGTGA

Antitoxin


Download         Length: 116 a.a.        Molecular weight: 13066.02 Da        Isoelectric Point: 7.0134

>AT42802 WP_033146339.1 NZ_AP026886:3842520-3842867 [Enterobacter asburiae]
MTLQQALRKPTTPGDVLQYEYLEPLNLKISDLAEMLNVHRNTISALVNNNRKLTADMAIKLAKAFDTTIEFWLNLQLNVD
IWEAQSNPRTQEELSRIKTVADVMAKRKSGQPDVA

Download         Length: 348 bp

>AT42802 NZ_AP026886:3842520-3842867 [Enterobacter asburiae]
ATGACACTTCAACAGGCACTCCGCAAACCCACCACGCCGGGCGACGTGTTGCAGTATGAGTATCTTGAACCGCTCAATCT
GAAAATCAGCGATCTGGCGGAGATGCTCAATGTTCACCGCAACACCATCAGCGCGCTGGTCAATAACAATCGCAAGCTTA
CTGCCGATATGGCGATCAAACTGGCAAAAGCCTTTGATACCACGATTGAATTTTGGCTTAACTTACAGCTGAACGTCGAT
ATCTGGGAAGCGCAATCCAACCCCAGGACGCAGGAGGAGCTAAGCCGTATAAAGACCGTTGCGGACGTCATGGCTAAGCG
AAAATCTGGCCAGCCGGATGTAGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A808IF03

Antitoxin

Source ID Structure
AlphaFold DB A0A808ICP3

References