Detailed information of TA system TA042801

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3198732..3199392
• Replicon: chromosome
• Accession: NZ_AP026886
• Organism: Enterobacter asburiae strain KAM576

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: OO861_RS15775
• Protein ID: WP_193139527.1
• Coordinates: 3199039..3199392 (-)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: OO861_RS15770
• Protein ID: WP_058841454.1
• Coordinates: 3198732..3199034 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OO861_RS15735 (KAM576c_30890)	3193894..3194406	-	513	WP_081178146.1	TIR domain-containing protein	-
OO861_RS15740 (KAM576c_30900)	3194448..3195059	-	612	WP_081178148.1	TIR domain-containing protein	-
OO861_RS15745 (KAM576c_30910)	3195059..3195787	-	729	WP_032454839.1	hypothetical protein	-
OO861_RS15750 (KAM576c_30920)	3196325..3196657	-	333	WP_064672767.1	type II toxin-antitoxin system PemK/MazF family toxin	-
OO861_RS15755 (KAM576c_30930)	3196658..3196915	-	258	WP_033145928.1	antitoxin	-
OO861_RS15760 (KAM576c_30940)	3197207..3197596	-	390	WP_059445031.1	RidA family protein	-
OO861_RS15765	3197738..3198657	+	920	Protein_3093	LysR family transcriptional regulator	-
OO861_RS15770 (KAM576c_30970)	3198732..3199034	-	303	WP_058841454.1	XRE family transcriptional regulator	Antitoxin
OO861_RS15775 (KAM576c_30980)	3199039..3199392	-	354	WP_193139527.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
OO861_RS15780 (KAM576c_30990)	3199570..3200463	-	894	WP_193139529.1	LysR substrate-binding domain-containing protein	-
OO861_RS15785 (KAM576c_31000)	3200634..3201242	+	609	WP_143346794.1	glutathione S-transferase	-
OO861_RS15790 (KAM576c_31010)	3201286..3202236	-	951	WP_023336219.1	HTH-type transcriptional regulator Cbl	-
OO861_RS15795 (KAM576c_31020)	3202333..3203250	-	918	WP_008499475.1	nitrogen assimilation transcriptional regulator NAC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• HigA2-like, helix-turn-helix domain (25-89)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13538.31 Da
• Isoelectric Point: 7.1678

>T42801 WP_193139527.1 NZ_AP026886:c3199392-3199039 [Enterobacter asburiae]
VWAINTTDRFDRWFTSLDDTDRASVLAALFVLREKGPGLSRPYADTLKGSRHSNMKELRIQSKGDPLRAFFAFDPNRTGI
VLCAGNKIGNERRFYDEMLLVADQEFTHWLDTLKERE

Nucleotide

• Length: 354 bp

>T42801 NZ_AP026886:c3199392-3199039 [Enterobacter asburiae]
GTGTGGGCAATTAACACGACGGACAGGTTTGATCGCTGGTTCACTTCGCTTGACGATACCGATCGCGCCAGCGTACTTGC
AGCACTGTTTGTTTTGCGAGAGAAGGGGCCGGGTTTATCGAGACCCTACGCTGATACGCTTAAAGGCTCTCGTCACAGCA
ACATGAAAGAGCTACGTATTCAGAGTAAGGGCGATCCCTTGCGGGCGTTTTTCGCCTTTGATCCAAACCGCACGGGTATC
GTGCTTTGTGCGGGCAATAAGATCGGCAATGAAAGGCGATTCTACGATGAGATGCTTCTCGTTGCTGACCAGGAATTCAC
TCACTGGCTGGATACATTAAAAGAGAGGGAATAG

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11016.82 Da
• Isoelectric Point: 5.7316

>AT42801 WP_058841454.1 NZ_AP026886:c3199034-3198732 [Enterobacter asburiae]
MGRTLEQLIADEKPEVVASAQAIATDILLNIHLAELREKVQKTQVEMAQALGIRQPTVAVMEKPGRDLKLSSLKRYVEAA
GGKLRLDVELPDGSHYELVL

Nucleotide

• Length: 303 bp

>AT42801 NZ_AP026886:c3199034-3198732 [Enterobacter asburiae]
ATGGGAAGAACCCTTGAGCAGCTTATAGCGGATGAAAAGCCGGAGGTTGTCGCCAGCGCTCAGGCGATAGCAACGGATAT
TTTGCTCAACATTCATCTTGCTGAGTTACGCGAGAAAGTACAAAAAACGCAGGTAGAAATGGCGCAGGCGTTGGGTATCA
GGCAACCTACCGTTGCGGTAATGGAAAAGCCCGGGCGCGATTTGAAACTCTCTTCTCTCAAGCGCTACGTTGAGGCGGCG
GGCGGAAAGCTGCGGCTTGATGTGGAGCTGCCGGACGGCTCGCACTATGAGTTAGTGCTGTAG