Detailed information of TA system TA042750

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 43525..43854
• Replicon: plasmid pIPCEC48_1
• Accession: NZ_AP026795
• Organism: Escherichia coli strain IPCEC48

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: OPA53_RS25720
• Protein ID: WP_001372321.1
• Coordinates: 43525..43650 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	43727..43854 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OPA53_RS25675 (38638)	38638..38865	-	228	WP_001254388.1	conjugal transfer relaxosome protein TraY	-
OPA53_RS25680 (38953)	38953..39630	-	678	WP_001348626.1	PAS domain-containing protein	-
OPA53_RS25685 (39764)	39764..40147	-	384	WP_001151566.1	conjugal transfer relaxosome DNA-binding protein TraM	-
OPA53_RS25690 (40477)	40477..41079	+	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
OPA53_RS25695 (41376)	41376..42197	-	822	WP_001234469.1	DUF932 domain-containing protein	-
OPA53_RS25700 (42316)	42316..42603	-	288	WP_000107535.1	hypothetical protein	-
OPA53_RS25705 (42628)	42628..42834	-	207	WP_000275859.1	hypothetical protein	-
OPA53_RS25710 (42904)	42904..43077	+	174	Protein_53	hypothetical protein	-
OPA53_RS25715 (43075)	43075..43305	-	231	WP_001426396.1	hypothetical protein	-
OPA53_RS25720 (43525)	43525..43650	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
OPA53_RS25725 (43592)	43592..43741	-	150	Protein_56	plasmid maintenance protein Mok	-
- (43727)	43727..43854	-	128	NuclAT_0	-	Antitoxin
- (43727)	43727..43854	-	128	NuclAT_0	-	Antitoxin
- (43727)	43727..43854	-	128	NuclAT_0	-	Antitoxin
- (43727)	43727..43854	-	128	NuclAT_0	-	Antitoxin
- (45296)	45296..45398	-	103	NuclAT_1	-	-
- (45296)	45296..45398	-	103	NuclAT_1	-	-
- (45296)	45296..45398	-	103	NuclAT_1	-	-
- (45296)	45296..45398	-	103	NuclAT_1	-	-
OPA53_RS25735 (45367)	45367..46129	-	763	Protein_58	plasmid SOS inhibition protein A	-
OPA53_RS25740 (46126)	46126..46560	-	435	WP_000845949.1	conjugation system SOS inhibitor PsiB	-
OPA53_RS25745 (46615)	46615..48573	-	1959	WP_042045562.1	ParB/RepB/Spo0J family partition protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / mph(A) / sul1 / qacE / aadA5 / blaCTX-M-15 / blaOXA-1 / aac(6')-Ib-cr	senB	1..165477	165477

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T42750 WP_001372321.1 NZ_AP026795:c43650-43525 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T42750 NZ_AP026795:c43650-43525 [Escherichia coli]
GTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACGAAAATCGCTGTGCGAGATTCGTTACAGAGACGG
ACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 128 bp

>AT42750 NZ_AP026795:c43854-43727 [Escherichia coli]
CGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGAAGATAGCCCCGTAGTAA
GTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin