Detailed information of TA system TA042749

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4987906..4988508
• Replicon: chromosome
• Accession: NZ_AP026794
• Organism: Escherichia coli strain IPCEC48

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: OPA53_RS24425
• Protein ID: WP_000897302.1
• Coordinates: 4988197..4988508 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: OPA53_RS24420
• Protein ID: WP_000356397.1
• Coordinates: 4987906..4988196 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OPA53_RS24390 (4983213)	4983213..4984142	+	930	WP_000027696.1	formate dehydrogenase accessory protein FdhE	-
OPA53_RS24395 (4984324)	4984324..4984566	-	243	WP_001068514.1	CopG family transcriptional regulator	-
OPA53_RS24400 (4984856)	4984856..4985704	+	849	WP_115800220.1	hypothetical protein	-
OPA53_RS24405 (4985729)	4985729..4986469	+	741	WP_000608806.1	hypothetical protein	-
OPA53_RS24410 (4986654)	4986654..4986872	-	219	WP_001251293.1	CopG family transcriptional regulator	-
OPA53_RS24415 (4987269)	4987269..4987547	-	279	WP_001296612.1	hypothetical protein	-
OPA53_RS24420 (4987906)	4987906..4988196	-	291	WP_000356397.1	NadS family protein	Antitoxin
OPA53_RS24425 (4988197)	4988197..4988508	-	312	WP_000897302.1	hypothetical protein	Toxin
OPA53_RS24430 (4988737)	4988737..4989645	+	909	WP_001385591.1	alpha/beta hydrolase	-
OPA53_RS24435 (4989709)	4989709..4990650	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
OPA53_RS24440 (4990695)	4990695..4991132	-	438	WP_000560981.1	D-aminoacyl-tRNA deacylase	-
OPA53_RS24445 (4991129)	4991129..4992001	-	873	WP_000920762.1	virulence factor BrkB family protein	-
OPA53_RS24450 (4991995)	4991995..4992594	-	600	WP_001296610.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T42749 WP_000897302.1 NZ_AP026794:c4988508-4988197 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T42749 NZ_AP026794:c4988508-4988197 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT42749 WP_000356397.1 NZ_AP026794:c4988196-4987906 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT42749 NZ_AP026794:c4988196-4987906 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: S1P416