Detailed information of TA system TA042729

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 1941139..1941641
• Replicon: chromosome
• Accession: NZ_AP026794
• Organism: Escherichia coli strain IPCEC48

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: E2QNP2
• Locus tag: OPA53_RS09185
• Protein ID: WP_000767819.1
• Coordinates: 1941387..1941641 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: E2QNP3
• Locus tag: OPA53_RS09180
• Protein ID: WP_001259253.1
• Coordinates: 1941139..1941390 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OPA53_RS09155 (1936317)	1936317..1937384	-	1068	WP_000080057.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-
OPA53_RS09160 (1937384)	1937384..1938454	-	1071	WP_000108967.1	histidinol-phosphate transaminase	-
OPA53_RS09165 (1938451)	1938451..1939755	-	1305	WP_001296211.1	histidinol dehydrogenase	-
OPA53_RS09170 (1939761)	1939761..1940660	-	900	WP_000131782.1	ATP phosphoribosyltransferase	-
OPA53_RS09175 (1940806)	1940806..1940856	-	51	WP_001364200.1	his operon leader peptide	-
OPA53_RS09180 (1941139)	1941139..1941390	+	252	WP_001259253.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
OPA53_RS09185 (1941387)	1941387..1941641	+	255	WP_000767819.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
OPA53_RS09190 (1941724)	1941724..1942548	+	825	WP_000754737.1	SDR family oxidoreductase	-
OPA53_RS09195 (1942594)	1942594..1943523	+	930	WP_000803366.1	LysR substrate-binding domain-containing protein	-
OPA53_RS09200 (1943738)	1943738..1943800	+	63	WP_010723108.1	membrane protein YoeI	-
OPA53_RS09205 (1943790)	1943790..1945148	+	1359	WP_000019194.1	putrescine/proton symporter PlaP	-
OPA53_RS09210 (1945365)	1945365..1945823	+	459	WP_001531805.1	IS200/IS605-like element IS200C family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1945365..1945823	458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10110.53 Da
• Isoelectric Point: 7.2749

>T42729 WP_000767819.1 NZ_AP026794:1941387-1941641 [Escherichia coli]
VKLIWSEESWDDYLYWQEADKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHLLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T42729 NZ_AP026794:1941387-1941641 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAGCAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAACTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCTTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9365.62 Da
• Isoelectric Point: 4.6127

>AT42729 WP_001259253.1 NZ_AP026794:1941139-1941390 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSDKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT42729 NZ_AP026794:1941139-1941390 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGACAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: E2QNP2

Antitoxin

• AlphaFold DB: E2QNP3