Detailed information of TA system TA042718

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 161719..161958
• Replicon: plasmid pIPCEC42_1
• Accession: NZ_AP026789
• Organism: Escherichia coli strain IPCEC42

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A762TWR7
• Locus tag: OPA56_RS26380
• Protein ID: WP_023144756.1
• Coordinates: 161824..161958 (+)
• Length: 45 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	161719..161779 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OPA56_RS26360 (159249)	159249..159995	+	747	WP_000205718.1	conjugal transfer pilus acetylase TraX	-
OPA56_RS26365 (160050)	160050..160610	+	561	WP_000139341.1	fertility inhibition protein FinO	-
OPA56_RS26370 (160741)	160741..160953	+	213	WP_013023861.1	hypothetical protein	-
OPA56_RS26375 (161466)	161466..161752	+	287	Protein_185	DUF2726 domain-containing protein	-
- (161719)	161719..161779	-	61	NuclAT_2	-	Antitoxin
- (161719)	161719..161779	-	61	NuclAT_2	-	Antitoxin
- (161719)	161719..161779	-	61	NuclAT_2	-	Antitoxin
- (161719)	161719..161779	-	61	NuclAT_2	-	Antitoxin
OPA56_RS26380 (161824)	161824..161958	+	135	WP_023144756.1	Hok/Gef family protein	Toxin
OPA56_RS26385 (162387)	162387..163133	-	747	WP_001016257.1	IS21-like element ISEc12 family helper ATPase IstB	-
OPA56_RS26390 (163148)	163148..164689	-	1542	WP_001298859.1	IS21-like element ISEc12 family transposase	-
OPA56_RS26395 (164880)	164880..165095	+	216	Protein_189	replication regulatory protein RepA	-
OPA56_RS26400 (165201)	165201..165332	+	132	Protein_190	protein CopA/IncA	-
OPA56_RS26405 (165332)	165332..165406	+	75	WP_031943482.1	RepA leader peptide Tap	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / mph(A) / sul1 / qacE / aadA5 / blaCTX-M-15 / blaOXA-1 / aac(6')-Ib-cr	senB	1..165449	165449

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-44)

Sequences

Toxin

Protein

• Length: 45 a.a.
• Molecular weight: 4896.90 Da
• Isoelectric Point: 7.7209

>T42718 WP_023144756.1 NZ_AP026789:161824-161958 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFREQLCELNIHRGNTVVQVTLA

Nucleotide

• Length: 135 bp

>T42718 NZ_AP026789:161824-161958 [Escherichia coli]
ATGACGAAATATACCCTTATCGGGTTGCTTGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACAGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTGA

Antitoxin

Nucleotide

• Length: 61 bp

>AT42718 NZ_AP026789:c161779-161719 [Escherichia coli]
TAAAGTGCATCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A762TWR7

Antitoxin