Detailed information of TA system TA042713

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4983344..4983946
• Replicon: chromosome
• Accession: NZ_AP026788
• Organism: Escherichia coli strain IPCEC42

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: OPA56_RS24430
• Protein ID: WP_000897302.1
• Coordinates: 4983635..4983946 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: OPA56_RS24425
• Protein ID: WP_000356397.1
• Coordinates: 4983344..4983634 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OPA56_RS24395 (4978651)	4978651..4979580	+	930	WP_000027696.1	formate dehydrogenase accessory protein FdhE	-
OPA56_RS24400 (4979762)	4979762..4980004	-	243	WP_001068514.1	CopG family transcriptional regulator	-
OPA56_RS24405 (4980294)	4980294..4981142	+	849	WP_115800220.1	hypothetical protein	-
OPA56_RS24410 (4981167)	4981167..4981907	+	741	WP_000608806.1	hypothetical protein	-
OPA56_RS24415 (4982092)	4982092..4982310	-	219	WP_001251293.1	CopG family transcriptional regulator	-
OPA56_RS24420 (4982707)	4982707..4982985	-	279	WP_001296612.1	hypothetical protein	-
OPA56_RS24425 (4983344)	4983344..4983634	-	291	WP_000356397.1	NadS family protein	Antitoxin
OPA56_RS24430 (4983635)	4983635..4983946	-	312	WP_000897302.1	hypothetical protein	Toxin
OPA56_RS24435 (4984175)	4984175..4985083	+	909	WP_001385591.1	alpha/beta hydrolase	-
OPA56_RS24440 (4985147)	4985147..4986088	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
OPA56_RS24445 (4986133)	4986133..4986570	-	438	WP_000560981.1	D-aminoacyl-tRNA deacylase	-
OPA56_RS24450 (4986567)	4986567..4987439	-	873	WP_000920762.1	virulence factor BrkB family protein	-
OPA56_RS24455 (4987433)	4987433..4988032	-	600	WP_001296610.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T42713 WP_000897302.1 NZ_AP026788:c4983946-4983635 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T42713 NZ_AP026788:c4983946-4983635 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT42713 WP_000356397.1 NZ_AP026788:c4983634-4983344 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT42713 NZ_AP026788:c4983634-4983344 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: S1P416