Detailed information of TA system TA042663

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1090646..1091373
• Replicon: chromosome
• Accession: NZ_AP026783
• Organism: Escherichia coli strain IPCEC31

Toxin (Protein)

• Gene name: higB
• Uniprot ID: J7Q991
• Locus tag: OO978_RS05295
• Protein ID: WP_000547564.1
• Coordinates: 1090646..1090957 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: OO978_RS05300
• Protein ID: WP_000126294.1
• Coordinates: 1090954..1091373 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OO978_RS05265 (1085788)	1085788..1087497	+	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-
OO978_RS05270 (1087507)	1087507..1088049	+	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
OO978_RS05275 (1088049)	1088049..1088816	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
OO978_RS05280 (1088813)	1088813..1089223	+	411	WP_001291921.1	formate hydrogenlyase assembly protein HycH	-
OO978_RS05285 (1089216)	1089216..1089686	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
OO978_RS05290 (1089711)	1089711..1090472	+	762	WP_001026446.1	hypothetical protein	-
OO978_RS05295 (1090646)	1090646..1090957	+	312	WP_000547564.1	type II toxin-antitoxin system HigB family toxin	Toxin
OO978_RS05300 (1090954)	1090954..1091373	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
OO978_RS05305 (1091487)	1091487..1092911	-	1425	WP_000110363.1	6-phospho-beta-glucosidase AscB	-
OO978_RS05310 (1092920)	1092920..1094377	-	1458	WP_001107861.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
OO978_RS05315 (1094637)	1094637..1095647	+	1011	WP_001392554.1	DNA-binding transcriptional regulator AscG	-
OO978_RS05320 (1095796)	1095796..1096323	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12426.13 Da
• Isoelectric Point: 9.7248

>T42663 WP_000547564.1 NZ_AP026783:1090646-1090957 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T42663 NZ_AP026783:1090646-1090957 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT42663 WP_000126294.1 NZ_AP026783:1090954-1091373 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT42663 NZ_AP026783:1090954-1091373 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCGGTT
GGCGTGGCGTTACTTCGCACGTTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A0A0FJG5